CA14 Protein (AA 19-290) (His tag)
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- Target See all CA14 Proteins
- CA14 (Carbonic Anhydrase XIV (CA14))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-290
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CA14 protein is labelled with His tag.
- Purpose
- Recombinant Human Carbonic Anhydrase 14/CA14 (N-6His)
- Sequence
- MNHKVHHHHH HMGQHWTYEG PHGQDHWPAS YPECGNNAQS PIDIQTDSVT FDPDLPALQP HGYDQPGTEP LDLHNNGHTV QLSLPSTLYL GGLPRKYVAA QLHLHWGQKG SPGGSEHQIN SEATFAELHI VHYDSDSYDS LSEAAERPQG LAVLGILIEV GETKNIAYEH ILSHLHEVRH KDQKTSVPPF NLRELLPKQL GQYFRYNGSL TTPPCYQSVL WTVFYRRSQI SMEQLEKLQG TLFSTEEEPS KLLVQNYRAL QPLNQRMVFA SFIQAGSSYT TGEMVDLQSR
- Characteristics
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Recombinant Human Carbonic Anhydrase 14/CA14 (N-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CA14 Protein
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Carbonic Anhydrase XIV (CA14) (AA 16-290) protein (His tag)
CA14 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Carbonic Anhydrase XIV (CA14) (AA 1-337) protein (GST tag)CA14 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Carbonic Anhydrase XIV (CA14) (AA 16-337) protein (rho-1D4 tag)Crystallography grade CA14 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Carbonic Anhydrase XIV (CA14) (AA 16-337) protein (rho-1D4 tag)Crystallography grade CA14 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Carbonic Anhydrase XIV (CA14) (AA 16-290) (Active) protein (His tag)CA14 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS Active
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 10 % Glycerol, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- CA14 (Carbonic Anhydrase XIV (CA14))
- Alternative Name
- Carbonic Anhydrase 14/CA14 (CA14 Products)
- Synonyms
- zgc:123188 Protein, AW536446 Protein, Ca14 Protein, CAXiV Protein, carbonic anhydrase XIV Protein, carbonic anhydrase 14 Protein, CA14 Protein, ca14 Protein, Car14 Protein
- Background
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Recombinant Human Carbonic Anhydrase 14/CA14 is produced by our E. coli expression system. The target protein is expressed with sequence (Gly19-Met290) of Human CA14 fused with a 6His tag at the N-terminus.
Carbonic Anhydrase 14 (CA14) belongs to the α-Carbonic Anhydrase family. It is highly expressed in all parts of the central nervous system and lowly expressed in adult liver, heart, small intestine, colon, kidney, urinary bladder, and skeletal muscle. CA14 along with other Carbonic Anhydrases (CAs) participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA14 is predicted to be a type I membrane protein and catalyzes the reversible hydration of carbon dioxide. - Molecular Weight
- 32.8 kDa
- UniProt
- Q9ULX7
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