UBE2V2 Protein (AA 1-145) (His tag)

Catalog No. ABIN1692317

Product Details

Target: UBE2V2 (Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2))

Protein Type: Recombinant

Protein Characteristics: AA 1-145

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2V2 protein is labelled with His tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 2/UBE2V2/DDVIT1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAVSTGVKVP RNFRLLEELE EGQKGVGDGT VSWGLEDDED MTLTRWTGMI IGPPRTNYEN RIYSLKVECG PKYPEAPPSV RFVTKINMNG INNSSGMVDA RSIPVLAKWQ NSYSIKVVLQ ELRRLMMSKE NMKLPQPPEG QTYNN

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 2/UBE2V2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn145) of Human UBE2V2 fused with a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50mm HEPES,150 mM NaCl, pH 7.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UBE2V2 (Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2))

Alternative Name: UBE2V2 (UBE2V2 Products)

Sub Type: Fusionprotein

Background: Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2) is an enzyme that belongs to the ubiquitin-conjugating enzyme family. UBE2V2 can be detected in the placenta, colon, liver, and skin. It forms a heterodimer with UBE2N. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains and which leads to protein degradation by the proteasome. UBE2V2 mediates transcriptional activation of target genes. It plays a role in the control of progress through the cell cycle and differentiation. It also plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 Variant 2, DDVit 1, Enterocyte Differentiation-Associated Factor 1, EDAF-1, Enterocyte Differentiation-Promoting Factor 1, EDPF-1, MMS2 Homolog, Vitamin D3-Inducible Protein, UBE2V2, MMS2, UEV2

Molecular Weight: 18.5 kDa

UniProt: Q15819

Pathways: Positive Regulation of Response to DNA Damage Stimulus