Calreticulin 3 Protein (CALR3) (AA 20-384)

Catalog No. ABIN1692465

Product Details

Target: Calreticulin 3 (CALR3)

Protein Type: Recombinant

Protein Characteristics: AA 20-384

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Calreticulin-3/CALR3/CRT2

Sequence: MTVYFQEEFL DGEHWRNRWL QSTNDSRFGH FRLSSGKFYG HKEKDKGLQT TQNGRFYAIS ARFKPFSNKG KTLVIQYTVK HEQKMDCGGG YIKVFPADID QKNLNGKSQY YIMFGPDICG FDIKKVHVIL HFKNKYHENK KLIRCKVDGF THLYTLILRP DLSYDVKIDG QSIESGSIEY DWNLTSLKKE TSPAESKDWE QTKDNKAQDW EKHFLDASTS KQSDWNGDLD GDWPAPMLQK PPYQDGLKPE GIHKDVWLHR KMKNTDYLTQ YDLSEFENIG AIGLELWQVR SGTIFDNFLI TDDEEYADNF GKATWGETKG PEREMDAIQA KEEMKKAREE EEEELLSGKI NRHEHYFNQF HRRNEL

Characteristics: Recombinant Human calreticulin-3/CALR3/CRT2 is produced with our E. coli expression system. The target protein is expressed with sequence (Thr20-Leu384) of Human CALR3 fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 5 months

Target Details

Target: Calreticulin 3 (CALR3)

Alternative Name: CALR3 (CALR3 Products)

Synonyms: 1700031L01Rik Protein, 6330586I20Rik Protein, Crt2 Protein, cspn Protein, CMH19 Protein, CRT2 Protein, CT93 Protein, A. thaliana calreticulin 3 Protein, AtCRT3 Protein, CALRETICULIN 3 Protein, EBS2 Protein, EMS-MUTAGENIZED BRI1 SUPPRESSOR 2 Protein, PRIORITY IN SWEET LIFE 1 Protein, PSL1 Protein, T27G7.13 Protein, T27G7_13 Protein, calreticulin 3 Protein, CALR Protein, calreticulin 3 Protein, CALR3 Protein, Calr3 Protein, CRT3 Protein

Sub Type: Fusionprotein

Background: Calreticulin-3 belongs to the calreticulin family, members of which are calcium binding chaperones localized mainly in the endoplasmic reticulum. It can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. During spermatogenesis process, Calreticulin-3 may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Defects in CALR3 are the cause of familial hypertrophic cardiomyopathy type 19 (CMH19), it is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain.
Alternative Names: Calreticulin-3, calreticulin-2, calsperin, CALR3, CRT2

Molecular Weight: 42.9 kDa

UniProt: Q96L12

Pathways: Activation of Innate immune Response