NECAP2 Protein (AA 1-263) (His tag)
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- Target See all NECAP2 Proteins
- NECAP2 (NECAP Endocytosis Associated 2 (NECAP2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-263
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NECAP2 protein is labelled with His tag.
- Purpose
- Recombinant Human Adaptin Ear-Binding Coat-Associated Protein 2/NECAP2 (N, C-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQFLEHHHHH H
- Characteristics
- Recombinant Human Adaptin ear-binding coat-associated protein 2/NECAP2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Phe263) of Human NECAP2 fused with a 6His tag at the N-terminus and C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product NECAP2 Protein
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NECAP Endocytosis Associated 2 (NECAP2) (AA 1-263) protein (His tag)
NECAP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
NECAP Endocytosis Associated 2 (NECAP2) (AA 1-263) protein (GST tag)NECAP2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
NECAP Endocytosis Associated 2 (NECAP2) (AA 1-266) protein (Strep Tag)Crystallography grade NECAP2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
NECAP Endocytosis Associated 2 (NECAP2) (AA 1-263) protein (Strep Tag)Crystallography grade NECAP2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
NECAP Endocytosis Associated 2 (NECAP2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NECAP2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
NECAP Endocytosis Associated 2 (NECAP2) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)NECAP2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- NECAP2 (NECAP Endocytosis Associated 2 (NECAP2))
- Alternative Name
- NECAP2 (NECAP2 Products)
- Synonyms
- MGC83534 Protein, 1110005F07Rik Protein, AA409105 Protein, C78898 Protein, NECAP endocytosis associated 2 Protein, NECAP endocytosis associated 2 L homeolog Protein, NECAP2 Protein, necap2.L Protein, necap2 Protein, Necap2 Protein
- Sub Type
- Fusionprotein
- Background
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NECAP2 belongs to the NECAP family. The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Adaptin ear-binding coat-associated protein 2 can interacts with AP1G1 and AP2A1 components of the adapter protein complex AP-1 and AP-2. It also interacts with the GAE domain proteins GGA1, GGA2 and GGA3.
Alternative Names: Adaptin ear-binding coat-associated protein 2, NECAP endocytosis associated protein 2, NECAP2 - Molecular Weight
- 31.5 kDa
- UniProt
- Q9NVZ3
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