ACAT2 Protein (AA 1-397) (His tag,TRX tag)

Catalog No. ABIN1692521

Product Details

Target: ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))

Protein Type: Recombinant

Protein Characteristics: AA 1-397

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ACAT2 protein is labelled with His tag,TRX tag.

Purpose: Recombinant Human Sterol O-Acyltransferase 2/SOAT2/ACAT2 (N-Trx, 6His)

Sequence: MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM ADIGSMNAGS DPVVIVSAAR TIIGSFNGAL AAVPVQDLGS TVIKEVLKRA TVAPEDVSEV IFGHVLAAGC GQNPVRQASV GAGIPYSVPA WSCQMICGSG LKAVCLAVQS IGIGDSSIVV AGGMENMSKA PHLAYLRTGV KIGEMPLTDS ILCDGLTDAF HNCHMGITAE NVAKKWQVSR EDQDKVAVLS QNRTENAQKA GHFDKEIVPV LVSTRRGLIE VKTDEFPRHG SNIEAMSKLK PYFLTDGTGT VTPANASGIN DGAAAVVLMK KSEADKRGLT PLARIVSWSQ VGVEPSIMGI GPIPAIKQAV TKAGWSLEDV DIFEINEAFA AVSAAIVKEL GLNPEKVNIE GGAIALGHPL GASGCRILVT LLHTLERMGR SRGVAALCIG GGMGIAMCVQ RE

Characteristics: Recombinant Human Sterol O-acyltransferase 2/SOAT2/ACAT2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Glu397) of Human ACAT2.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))

Alternative Name: ACAT2 (ACAT2 Products)

Synonyms: fb10a06 Protein, fb53f08 Protein, wu:fb10a06 Protein, wu:fb53f08 Protein, AW742799 Protein, Tcp-1x Protein, Tcp1-rs1 Protein, Ab2-076 Protein, Acat3 Protein, EMB1276 Protein, EMBRYO DEFECTIVE 1276 Protein, MIF21.12 Protein, MIF21_12 Protein, acetoacetyl-CoA thiolase 2 Protein, acetyl-CoA acetyltransferase 2 Protein, acetyl-Coenzyme A acyltransferase 2 Protein, acetyl-Coenzyme A acetyltransferase 2 Protein, acetyl-CoA acetyltransferase 2 S homeolog Protein, acetoacetyl-CoA thiolase 2 Protein, ACAT2 Protein, CNA05060 Protein, acat2 Protein, Acat2 Protein, acat2.S Protein

Background: ACAT2 is a cytoplasmic enzyme which belongs to the thiolase family. ACAT2 takes part in lipid metabolism, lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. It is responsible for the synthesis of cholesteryl esters which are part of lipoproteins containing apoB. ACAT2 deficiency contributes to severe mental retardation and hypotonus.
Alternative Names: Acetyl-CoA acetyltransferase cytosolic, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase, ACTL, ACAT2

Molecular Weight: 59.1 kDa

UniProt: O75908