LMCD1 Protein (AA 1-365) (His tag)

Catalog No. ABIN1692525

Product Details

Target: LMCD1 (LIM and Cysteine-Rich Domains 1 (LMCD1))

Protein Type: Recombinant

Protein Characteristics: AA 1-365

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This LMCD1 protein is labelled with His tag.

Purpose: Recombinant Human LIM and Cysteine-Rich Domains Protein 1/LMCD1/Dyxin (N, C-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAKVAKDLNP GVKKMSLGQL QSARGVACLG CKGTCSGFEP HSWRKICKSC KCSQEDHCLT SDLEDDRKIG RLLMDSKYST LTARVKGGDG IRIYKRNRMI MTNPIATGKD PTFDTITYEW APPGVTQKLG LQYMELIPKE KQPVTGTEGA FYRRRQLMHQ LPIYDQDPSR CRGLLENELK LMEEFVKQYK SEALGVGEVA LPGQGGLPKE EGKQQEKPEG AETTAATTNG SLSDPSKEVE YVCELCKGAA PPDSPVVYSD RAGYNKQWHP TCFVCAKCSE PLVDLIYFWK DGAPWCGRHY CESLRPRCSG CDEIIFAEDY QRVEDLAWHR KHFVCEGCEQ LLSGRAYIVT KGQLLCPTCS KSKRSLEHHH HHH

Characteristics: Recombinant Human LIM and cysteine-rich domains protein 1/LMCD1/Dyxin is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ser365) of Human LMCD1 fused with a 6His tag at the N-terminus and a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: LMCD1 (LIM and Cysteine-Rich Domains 1 (LMCD1))

Alternative Name: LMCD1 (LMCD1 Products)

Synonyms: LMCD1 Protein, AW455500 Protein, AW553074 Protein, dyxin Protein, LIM and cysteine rich domains 1 Protein, LIM and cysteine-rich domains 1 Protein, LMCD1 Protein, lmcd1 Protein, Lmcd1 Protein

Sub Type: Fusionprotein

Background: LMCD1 is transcriptional cofactor which contains a cysteine-rich domain in the N-terminal region and 2 LIM domains in the C-terminal region. It also has several potential phosphorylation and N-myristoylation sites and a single potential N-glycosylation site. LMCD1 is expressed in many tissues with highest abundance in skeletal muscle. LMCD1 restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. It plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.
Alternative Names: LIM and cysteine-rich domains protein 1, LMCD1, Dyxin

Molecular Weight: 44 kDa

UniProt: Q9NZU5