PDIA3 Protein (AA 25-505) (His tag)

Catalog No. ABIN1880692

Product Details

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Protein Type: Recombinant

Protein Characteristics: AA 25-505

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This PDIA3 protein is labelled with His tag.

Purpose: Recombinant Human Protein Disulfide-Isomerase A3/PDIA3 (C-6His)

Sequence: SDVLELTDDN FESRISDTGS AGLMLVEFFA PWCGHCKRLA PEYEAAATRL KGIVPLAKVD CTANTNTCNK YGVSGYPTLK IFRDGEEAGA YDGPRTADGI VSHLKKQAGP ASVPLRTEEE FKKFISDKDA SIVGFFDDSF SEAHSEFLKA ASNLRDNYRF AHTNVESLVN EYDDNGEGII LFRPSHLTNK FEDKTVAYTE QKMTSGKIKK FIQENIFGIC PHMTEDNKDL IQGKDLLIAY YDVDYEKNAK GSNYWRNRVM MVAKKFLDAG HKLNFAVASR KTFSHELSDF GLESTAGEIP VVAIRTAKGE KFVMQEEFSR DGKALERFLQ DYFDGNLKRY LKSEPIPESN DGPVKVVVAE NFDEIVNNEN KDVLIEFYAP WCGHCKNLEP KYKELGEKLS KDPNIVIAKM DATANDVPSP YEVRGFPTIY FSPANKKLNP KKYEGGRELS DFISYLQREA TNPPVIQEEK PKKKKKAQED LVDHHHHHH

Characteristics: Recombinant Human Protein disulfide-isomerase A3/PDIA3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ser25-Leu505) of Human PDIA3 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,10 % Glycerol, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Alternative Name: PDIA3 (PDIA3 Products)

Synonyms: ER60 Protein, ERp57 Protein, ERp60 Protein, ERp61 Protein, GRP57 Protein, GRP58 Protein, HsT17083 Protein, P58 Protein, PI-PLC Protein, grp-58 Protein, 58kDa Protein, Erp Protein, Grp58 Protein, PDI Protein, PDI-Q2 Protein, PLC[a] Protein, Plca Protein, 1 Protein, 25D3-membrane-associated Protein, sb:cb825 Protein, erp57 Protein, grp58 Protein, pdia3 Protein, protein disulfide isomerase family A member 3 Protein, protein disulfide-isomerase A3 Protein, protein disulfide isomerase associated 3 Protein, protein disulfide isomerase family A, member 3 Protein, protein disulfide isomerase family A member 3 S homeolog Protein, PDIA3 Protein, Tsp_10062 Protein, Pdia3 Protein, pdia3 Protein, pdia3.S Protein

Sub Type: Fusionprotein

Background: PDIA3 protein is also known as Protein disulfide-isomerase A3. It is a protein that in humans is encoded by the PDIA3 gene.PDIA3 is an enzyme that belongs to the endoplasmic reticulum and interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. PDIA3 interacts with thiazide-sensitive sodium-chloride cotransporter in the kidney and is induced by glucose deprivation. PDIA3 is part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is important for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface.
Alternative Names: PDIA3 protein is also known as Protein disulfide-isomerase A3.

Molecular Weight: 55.3 kDa

UniProt: A5D7E8

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis