ITLN1/Omentin Protein (AA 19-298) (His tag)

Catalog No. ABIN1945367

Product Details

Target: ITLN1/Omentin (ITLN1) (Intelectin 1 (Galactofuranose Binding) (ITLN1))

Protein Type: Recombinant

Protein Characteristics: AA 19-298

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ITLN1/Omentin protein is labelled with His tag.

Purpose: Recombinant Human Omentin/Intelectin-1/ITLN-1 (N-6His)

Sequence: MNHKVHHHHH HMTDEANTYF KEWTCSSSPS LPRSCKEIKD ECPSAFDGLY FLRTENGVIY QTFCDMTSGG GGWTLVASVH ENDMRGKCTV GDRWSSQQGS KAVYPEGDGN WANYNTFGSA EAATSDDYKN PGYYDIQAKD LGIWHVPNKS PMQHWRNSSL LRYRTDTGFL QTLGHNLFGI YQKYPVKYGE GKCWTDNGPV IPVVYDFGDA QKTASYYSPY GQREFTAGFV QFRVFNNERA ANALCAGMRV TGCNTEHHCI GGGGYFPEAS PQQCGDFSGF DWSGYGTHVG YS

Characteristics: Recombinant Human Intelectin-1/Endothelial lectin HL-1/Galactofuranose-binding lectin is produced with our E. coli expression system. The target protein is expressed with sequence (AA 19-298) of human Intelectin-1 fused with a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mM Tris,100 mMNaCl, 5 mM GSH, 0.5 mM GSSG, pH 8.0 .

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ITLN1/Omentin (ITLN1) (Intelectin 1 (Galactofuranose Binding) (ITLN1))

Alternative Name: Intelectin-1 (ITLN1 Products)

Synonyms: IntL Protein, Itln Protein, Itln2 Protein, Itln5 Protein, Itlna Protein, Lfr Protein, HL-1 Protein, HL1 Protein, INTL Protein, ITLN Protein, LFR Protein, hIntL Protein, omentin Protein, LfR Protein, Xeel Protein, hl-1 Protein, hl1 Protein, intl Protein, itln Protein, itln1 Protein, lfr Protein, xeel Protein, INTL1 Protein, zgc:153267 Protein, intelectin 1 (galactofuranose binding) Protein, intelectin 1 Protein, intelectin 1 L homeolog Protein, Itln1 Protein, ITLN1 Protein, itln1.L Protein, itln1 Protein

Background: Intelectin-1(ITLN1) is a secreted protein and contains 1 fibrinogen C-terminal domain. Intelectin-1 is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The protein has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. It increases AKT phosphorylation in the absence and presence of insulin and it may play a role in the defense systemagainst microorganisms. It also may specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosy l residues, in a calcium-dependent manner.

Molecular Weight: 32.7 kDa

UniProt: Q8WWA0