CHRNB3 Protein (AA 25-232) (His tag)

Catalog No. ABIN1945433

Product Details

Target: CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))

Protein Type: Recombinant

Protein Characteristics: AA 25-232

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CHRNB3 protein is labelled with His tag.

Purpose: Recombinant Human Neuronal Acetylcholine Receptor Subunit β-3/CHRNB3 (C-6His)

Sequence: IAENEDALLR HLFQGYQKWV RPVLHSNDTI KVYFGLKISQ LVDVDEKNQL MTTNVWLKQE WTDHKLRWNP DDYGGIHSIK VPSESLWLPD IVLFENADGR FEGSLMTKVI VKSNGTVVWT PPASYKSSCT MDVTFFPFDR QNCSMKFGSW TYDGTMVDLI LINENVDRKD FFDNGEWEIL NAKGMKGNRR DGVYSYPFIT YSFVLRRLLD HHHHHH

Characteristics: Recombinant Human Neuronal acetylcholine receptor subunit beta-3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (AA 25-232) of Human CHRNB3 fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))

Alternative Name: CHRNB3 (CHRNB3 Products)

Synonyms: 5730417K16Rik Protein, Acrb3 Protein, NACHR Protein, CHRNB3 Protein, chrnb3 Protein, cholinergic receptor nicotinic beta 3 subunit Protein, neuronal acetylcholine receptor subunit beta-3 Protein, cholinergic receptor, nicotinic, beta polypeptide 3 Protein, cholinergic receptor, nicotinic beta 3 L homeolog Protein, cholinergic receptor, nicotinic beta 3 Protein, cholinergic receptor, nicotinic, beta 3b (neuronal) Protein, cholinergic receptor, nicotinic, beta polypeptide 3a Protein, CHRNB3 Protein, CpipJ_CPIJ012638 Protein, Chrnb3 Protein, chrnb3.L Protein, chrnb3 Protein, chrnb3b Protein, chrnb3a Protein

Background: Neuronal acetylcholine receptor subunit beta-3(CHRNB3) is a cell membrane protein and belongs to the ligand-gated ion channel (TC 1.A.9) family. CHRNB3 seems to be composed of two different type of subunits: alpha and beta. The CHRNB3 are (hetero) pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of CHRNB3 are encoded by separate genes and have different primary structure. There are several subtypes of neuronal CHRNB3 that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if like muscle alpha-1, they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits.

Molecular Weight: 25.3 kDa

UniProt: Q05901