Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2) (AA 1-413) (Active) protein (His tag)

Details for Product No. ABIN1981684, Supplier: Log in to see
Protein Name
  • KAT4
  • KATIV
  • mitAAT
  • AL022787
  • FABP-pm
  • Got-2
  • mAspAT
  • ASPATA
  • got2
  • zgc:66329
  • glutamic-oxaloacetic transaminase 2
  • glutamatic-oxaloacetic transaminase 2, mitochondrial
  • glutamic-oxaloacetic transaminase 2a, mitochondrial
  • glutamic-oxaloacetic transaminase 2 L homeolog
  • GOT2
  • got2
  • Got2
  • got2a
  • got2.L
Protein Characteristics
AA 1-413
8
2
1
1
1
1
1
1
1
Origin
Human
11
3
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
10
7
2
2
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
SDS-PAGE (SDS)
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Sequence MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK
Characteristics Molecule Information: 48.4 kDa (433 aa, 1-413 aa + His Tag)
Purity ≥95 % by SDS-PAGE
Biological Activity Comment Specific activity is > 44 unit/mg, in which one unit will convert 1.0 μmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.
Unit Definition One unit will convert 1.0 μmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.
Background GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternative Names: Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT
Molecular Weight 48.4 kDa
Gene ID 14719
UniProt P05202
Research Area Cancer, Metabolism, Amino Acids
Pathways Monocarboxylic Acid Catabolic Process
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL solution 20 mM Tris-HCl buffer ( pH 8.0), 10 % glycerol, 150 mM NaCl and 1 mM DTT.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2) (AA 1-413) (Active) protein (His tag) (ABIN1981684) Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2) (AA 1-413) (Active) protein (His tag)
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