Interleukin 12b (IL12B) (AA 1-328), (Subunit p40) (Active) protein (Fc Tag)

Details for Product No. ABIN2001973, Supplier: Log in to see
Protein Name
  • IL-12B
  • IL12B
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL12p40
  • IL-12
  • IL-12.p40
  • IL-12p40
  • interleukin 12 p40 subunit
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12b
  • interleukin 12B
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • IL-12
  • IL12A
  • Il12b
  • IL12B
Protein Characteristics
AA 1-328, Subunit p40
14
9
5
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
Origin
Human
33
13
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Source
Human Cells
21
15
8
7
3
3
2
2
2
2
2
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This IL12B protein is labelled with Fc Tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The recombinant human IL12B consists of 544 amino acids and has a predicted molecular mass of 61.4 kDa.

Protein Structure: A DNA sequence encoding the p40 subunit of human IL12, termed asIL12B (NP_002178.2) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus.
Predicted N-Term: Ile 23
Purity > 85 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Measured by its binding ability in a functional ELISA . Immobilized human IL12RB1 at 20 μg/ml (100 μl/well) can bind human IL12B with a linear ranger of 2.56-64 ng/ml.
Background Synonyms: CLMF,CLMF2,IL-12B,IMD28,IMD29,NKSF,NKSF2
Molecular Weight 61.4 kDa
NCBI Accession NP_002178
Research Area Hormones, Cytokines
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Interleukin 12b (IL12B) (AA 1-328), (Subunit p40) (Active) protein (Fc Tag) (ABIN2001973) Interleukin 12b (IL12B) (AA 1-328), (Subunit p40) (Active) protein (Fc Tag)
Background publications Kim, Loveland, Sitz, Ratto Kim, Mclinden, Tencer, Davis, Burke, Boswell, Redfield, Birx: "Expansion of restricted cellular immune responses to HIV-1 envelope by vaccination: IL-7 and IL-12 differentially augment cellular proliferative responses to HIV-1." in: Clinical and experimental immunology, Vol. 108, Issue 2, pp. 243-50, 1997 (PubMed).

Thierfelder, van Deursen, Yamamoto, Tripp, Sarawar, Carson, Sangster, Vignali, Doherty, Grosveld, Ihle: "Requirement for Stat4 in interleukin-12-mediated responses of natural killer and T cells." in: Nature, Vol. 382, Issue 6587, pp. 171-4, 1996 (PubMed).

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, 1995

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, 1995 (PubMed).

Sieburth, Jabs, Warrington, Li, Lasota, LaForgia, Kelleher, Huebner, Wasmuth, Wolf: "Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5." in: Genomics, Vol. 14, Issue 1, pp. 59-62, 1992 (PubMed).

Gearing, Cosman: "Homology of the p40 subunit of natural killer cell stimulatory factor (NKSF) with the extracellular domain of the interleukin-6 receptor." in: Cell, Vol. 66, Issue 1, pp. 9-10, 1991 (PubMed).