Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B) (AA 1-401) (Active) protein (His tag)

Details for Product No. ABIN2002484, Supplier: Log in to see
Protein Name
  • OCIF
  • OPG
  • TR1
  • Opg
  • ocif
  • opg
  • tr1
  • TNF receptor superfamily member 11b
  • tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
  • TNF receptor superfamily member 11B
  • tumor necrosis factor receptor superfamily member 11b S homeolog
  • tumor necrosis factor receptor superfamily, member 11b
  • TNFRSF11B
  • Tnfrsf11b
  • tnfrsf11b.S
Protein Characteristics
AA 1-401
12
2
1
1
1
1
1
1
Origin
Human
28
4
1
1
1
Source
Human Cells
9
8
4
3
2
2
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Characteristics The secreted recombinant human TNFRSF11B comprises 391 amino acids and has a predicted molecular mass of 45.3 kDa. As a result of glycosylation, rhTNFRSF11B migrates as an approximately 55 kDa bnd in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human TNFRSF11B (NP_002537.3) (Met 1-Leu 401) was fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Glu 22
Purity > 97 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment 1. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 5-20 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10 .
2. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF11B-His at 10 μg/ml (100 μl/well) can bind human Fc-TNFSF11 with a linear ranger of 3.125-200 ng/mL.
Background Synonyms: MGC29565,OCIF,OPG,PDB5,TR1
Molecular Weight 45.3 kDa, 55 kDa
NCBI Accession NP_002537
Research Area Organogenesis, Innate Immunity, Cytokines
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B) (AA 1-401) (Active) protein (His tag) (ABIN2002484) Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B) (AA 1-401) (Active) protein (His tag)
Background publications Zhang, Henzel: "Signal peptide prediction based on analysis of experimentally verified cleavage sites." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 10, pp. 2819-24, 2004

Zhang, Henzel: "Signal peptide prediction based on analysis of experimentally verified cleavage sites." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 10, pp. 2819-24, 2004 (PubMed).

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Yasuda, Shima, Nakagawa, Mochizuki, Yano, Fujise, Sato, Goto, Yamaguchi, Kuriyama, Kanno, Murakami, Tsuda, Morinaga, Higashio: "Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): a mechanism by which OPG/OCIF inhibits osteoclastogenesis in vitro." in: Endocrinology, Vol. 139, Issue 3, pp. 1329-37, 1998 (PubMed).

Simonet, Lacey, Dunstan, Kelley, Chang, Lüthy, Nguyen, Wooden, Bennett, Boone, Shimamoto, DeRose, Elliott, Colombero, Tan, Trail, Sullivan, Davy, Bucay, Renshaw-Gegg, Hughes, Hill, Pattison, Campbell et al.: "Osteoprotegerin: a novel secreted protein involved in the regulation of bone density. ..." in: Cell, Vol. 89, Issue 2, pp. 309-19, 1997 (PubMed).

Did you look for something else?