Protease, serine, 8 (PRSS8) (AA 30-322), (Mature) (Active) protein (His tag)

Details for Product No. ABIN2002505, Supplier: Log in to see
Protein Name
  • CAP1
  • PROSTASIN
  • prostasin
  • 2410039E18Rik
  • AI313909
  • C79772
  • fr
  • mCAP1
  • protease, serine, 8
  • protease, serine, 8 (prostasin)
  • Prss8
  • PRSS8
Protein Characteristics
AA 30-322, Mature
3
2
1
1
1
1
1
1
1
1
1
1
Origin
Human
6
5
3
Source
Human Cells
7
4
2
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The mature recombinant human Prss8 consists of 304 amino acids and predicts a molecular mass of 32.8 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhPrss8 is approximately 40 kDa due to glycosylation.

Protein Structure: A DNA sequence encoding the mature form of human Prss8 (NP_002764.1) (Ala 30-Arg 322) was fused with a signal peptide at the N-terminus and a polyhistidine-tag at the C-terminus.
Predicted N-Term: Ala 30
Purity > 97 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC . The specific activity is >10 pmoles/min/μg.
Background Synonyms: CAP1,PROSTASIN
Molecular Weight 32.8 kDa, 40 kDa
NCBI Accession NP_002764
Research Area Proteolysis / Ubiquitin
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Protease, serine, 8 (PRSS8) (AA 30-322), (Mature) (Active) protein (His tag) (ABIN2002505) Protease, serine, 8 (PRSS8) (AA 30-322), (Mature) (Active) protein (His tag)
Background publications Verghese, Gutknecht, Caughey: "Prostasin regulates epithelial monolayer function: cell-specific Gpld1-mediated secretion and functional role for GPI anchor." in: American journal of physiology. Cell physiology, Vol. 291, Issue 6, pp. C1258-70, 2006

Verghese, Gutknecht, Caughey: "Prostasin regulates epithelial monolayer function: cell-specific Gpld1-mediated secretion and functional role for GPI anchor." in: American journal of physiology. Cell physiology, Vol. 291, Issue 6, pp. C1258-70, 2006 (PubMed).

Chen, Chai: "Prostasin serine protease inhibits breast cancer invasiveness and is transcriptionally regulated by promoter DNA methylation." in: International journal of cancer. Journal international du cancer, Vol. 97, Issue 3, pp. 323-9, 2002 (PubMed).

Chen, Skinner, Kauffman, Chao, Chao, Thaler, Chai: "Prostasin is a glycosylphosphatidylinositol-anchored active serine protease." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21434-42, 2001 (PubMed).

Yu, Chao, Chao: "Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue distribution, and localization in prostate gland." in: The Journal of biological chemistry, Vol. 269, Issue 29, pp. 18843-8, 1994 (PubMed).

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