Tissue Factor Pathway Inhibitor 2 (TFPI2) (AA 1-213) (Active) protein (His tag)

Details for Product No. ABIN2003028, Supplier: Log in to see
Protein Name
  • MGC68843
  • TFPI2
  • MGC108301
  • si:ch211-262k23.2
  • tfpi2
  • PP5
  • REF1
  • TFPI-2
  • AV000670
  • PP5/TFPI-2
  • tissue factor pathway inhibitor 2
  • Tissue factor pathway inhibitor 2
  • papilin-like
  • tissue factor pathway inhibitor 2-like
  • tfpi2
  • TFPI2
  • LOC100218198
  • LOC100718882
  • Tfpi2
  • LOC100520144
Protein Characteristics
AA 1-213
4
2
2
2
2
2
1
1
1
Origin
Human
17
5
3
2
Source
Human Cells
9
3
2
2
2
1
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This TFPI2 protein is labelled with His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Characteristics The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Asp 23
Purity > 95 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.
Background Synonyms: PP5,REF1,TFPI-2
Molecular Weight 23.2 kDa, 32-34 kDa
NCBI Accession NP_006519
Research Area Proteolysis / Ubiquitin
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Tissue Factor Pathway Inhibitor 2 (TFPI2) (AA 1-213) (Active) protein (His tag) (ABIN2003028) Tissue Factor Pathway Inhibitor 2 (TFPI2) (AA 1-213) (Active) protein (His tag)
Background publications Miyagi, Koshikawa, Yasumitsu, Miyagi, Hirahara, Aoki, Misugi, Umeda, Miyazaki: "cDNA cloning and mRNA expression of a serine proteinase inhibitor secreted by cancer cells: identification as placental protein 5 and tissue factor pathway inhibitor-2." in: Journal of biochemistry, Vol. 116, Issue 5, pp. 939-42, 1995

Miyagi, Koshikawa, Yasumitsu, Miyagi, Hirahara, Aoki, Misugi, Umeda, Miyazaki: "cDNA cloning and mRNA expression of a serine proteinase inhibitor secreted by cancer cells: identification as placental protein 5 and tissue factor pathway inhibitor-2." in: Journal of biochemistry, Vol. 116, Issue 5, pp. 939-42, 1995 (PubMed).