Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 1-282) (Active) protein (His tag)

Details for Product No. ABIN2003062, Supplier: Log in to see
Protein Name
  • A630013F22Rik
  • AW552122
  • EPI
  • LACI
  • TFI
  • TFPI1
  • tissue factor pathway inhibitor
  • tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
  • Tfpi
  • LOC100155068
  • TFPI
Protein Characteristics
AA 1-282
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Origin
Human
18
5
3
2
2
2
Source
Human Cells
15
6
3
2
2
2
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This TFPI protein is labelled with His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The secreted recombinant human TFPI comprises 265 amino acids with a predicted molecular mass of 30.6 kDa. As a result of glycosylation, the apparent molecular mass of rhTFPI is approximately 42-45 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Asp 29
Purity > 85 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5 .
Background Synonyms: EPI,LACI,TFI,TFPI1
Molecular Weight 30.6 kDa, 42-45 kDa
NCBI Accession NP_006278
Research Area Cardiovascular
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 1-282) (Active) protein (His tag) (ABIN2003062) Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 1-282) (Active) protein (His tag)
Product cited in: Marlu, Polack: "Gla-domainless factor Xa: molecular bait to bypass a blocked tenase complex." in: Haematologica, Vol. 97, Issue 8, pp. 1165-72, 2012 (PubMed).

Background publications Bajaj, Birktoft, Steer, Bajaj: "Structure and biology of tissue factor pathway inhibitor." in: Thrombosis and haemostasis, Vol. 86, Issue 4, pp. 959-72, 2001

Bajaj, Birktoft, Steer, Bajaj: "Structure and biology of tissue factor pathway inhibitor." in: Thrombosis and haemostasis, Vol. 86, Issue 4, pp. 959-72, 2001 (PubMed).

Li, Wun: "Proteolysis of tissue factor pathway inhibitor (TFPI) by plasmin: effect on TFPI activity." in: Thrombosis and haemostasis, Vol. 80, Issue 3, pp. 423-7, 1998 (PubMed).

Ruci?ska, Gacko, Skrzydlewski: "Tissue factor pathway inhibitor (TFPI)." in: Roczniki Akademii Medycznej w Bia?ymstoku (1995), Vol. 42 Suppl 1, pp. 118-22, 1997 (PubMed).

Broze, Warren, Novotny, Higuchi, Girard, Miletich: "The lipoprotein-associated coagulation inhibitor that inhibits the factor VII-tissue factor complex also inhibits factor Xa: insight into its possible mechanism of action." in: Blood, Vol. 71, Issue 2, pp. 335-43, 1988 (PubMed).

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