CA12 (Extracellular Domain), (AA 1-291) (Active) protein (His tag)

Details for Product No. ABIN2003146, Supplier: Log in to see
Protein Name
  • CAXII
  • HsT18816
  • CAR12
  • 2310047E01Rik
  • AI314958
  • Ca12
  • carbonic anhydrase XII
  • carbonic anyhydrase 12
  • CA12
  • Car12
Protein Characteristics
Extracellular Domain, AA 1-291
3
2
1
1
1
1
1
1
1
1
1
1
Origin
Human
7
6
2
1
Source
Human Cells
8
2
2
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The recombinant human CA12 consisting of 278 amino acids and has a calculated molecular mass of 31.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the extracellular domain of human CA12 (NP_001209.1) (Met 1-Gln 291) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Ala 25
Purity > 97 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Background Synonyms: CA12,CAXII,FLJ20151,HsT18816
Molecular Weight 31.6 kDa, 40-45 kDa
NCBI Accession NP_001209
Research Area Cancer
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for CA12 (Extracellular Domain), (AA 1-291) (Active) protein (His tag) (ABIN2003146) CA12 (Extracellular Domain), (AA 1-291) (Active) protein (His tag)
Product cited in: Lecoeur, Goossens, Vaccher, Bonte, Foulon: "A multivariate approach for the determination of isoelectric point of human carbonic anhydrase isoforms by capillary isoelectric focusing." in: Electrophoresis, Vol. 32, Issue 20, pp. 2857-66, 2011 (PubMed).

Background publications Ivanov, Kuzmin, Wei, Pack, Geil, Johnson, Stanbridge, Lerman: "Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 21, pp. 12596-601, 1998 (PubMed).