Serine/threonine-Protein Kinase MST4 (MST4) (AA 1-416) (Active) protein (GST tag)

Details for Product No. ABIN2003193, Supplier: Log in to see
Protein Name
  • Mst4
  • MASK
  • RGD1563568
  • serine/threonine kinase 26
  • Stk26
  • STK26
Protein Characteristics
AA 1-416
1
1
Origin
Human
3
Source
Baculovirus infected Insect Cells
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
GST tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The recombinant human MST41/GST chimera consists of 641 amino acids and predicts a molecular mass of 73 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human MST4 isoform 1 (NP_057626.2) (Met 1-Pro 416) was expressed with the fused GST tag at N-terminus.
Predicted N-Term: Met
Purity > 95 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment The specific activity was determined to be 15 nmol/min/mg using MBP as substrate.
Background Synonyms: MASK,MST4
Molecular Weight 73 kDa, 65 kDa
NCBI Accession NP_057626
Research Area Signaling, Tyrosine Kinases, Apoptosis/Necrosis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 25 % glycerol, 0.6 mM GSH, 0.5 mM PMSF, 0.5 mM EDTA, 2 mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Expiry Date 12 months
Supplier Images
 image for Serine/threonine-Protein Kinase MST4 (MST4) (AA 1-416) (Active) protein (GST tag) (ABIN2003193) Serine/threonine-Protein Kinase MST4 (MST4) (AA 1-416) (Active) protein (GST tag)
Background publications Ma, Zhao, Shan, Long, Chen, Chen, Zhang, Han, Ma: "PDCD10 interacts with Ste20-related kinase MST4 to promote cell growth and transformation via modulation of the ERK pathway." in: Molecular biology of the cell, Vol. 18, Issue 6, pp. 1965-78, 2007

Ma, Zhao, Shan, Long, Chen, Chen, Zhang, Han, Ma: "PDCD10 interacts with Ste20-related kinase MST4 to promote cell growth and transformation via modulation of the ERK pathway." in: Molecular biology of the cell, Vol. 18, Issue 6, pp. 1965-78, 2007 (PubMed).

Sung, Luo, Qian, Lee, Jallal, Gishizky: "The Ste20 kinase MST4 plays a role in prostate cancer progression." in: Cancer research, Vol. 63, Issue 12, pp. 3356-63, 2003 (PubMed).

Lin, Chen, Fang, Robinson, Kung, Shih: "MST4, a new Ste20-related kinase that mediates cell growth and transformation via modulating ERK pathway." in: Oncogene, Vol. 20, Issue 45, pp. 6559-69, 2001 (PubMed).

Qian, Lin, Espinosa, LeBeau, Rosner: "Cloning and characterization of MST4, a novel Ste20-like kinase." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22439-45, 2001 (PubMed).

Fedoroff: "Deletion mutants of Xenopus laevis 5S ribosomal DNA." in: Cell, Vol. 16, Issue 3, pp. 551-63, 1979 (PubMed).

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