Met Proto-Oncogene (MET) (Extracellular Domain), (AA 1-932) (Active) protein (Fc Tag,His tag)

Details for Product No. ABIN2003239, Supplier: Log in to see
Protein Name
  • AUTS9
  • HGFR
  • RCCP2
  • c-Met
  • AI838057
  • HGF
  • Par4
  • Hgfr
  • c-met
  • MET proto-oncogene, receptor tyrosine kinase
  • met proto-oncogene
  • MET
  • Met
Protein Characteristics
Extracellular Domain, AA 1-932
29
13
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Origin
Human
47
5
5
3
1
Source
Human Cells
21
17
10
8
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This MET protein is labelled with Fc Tag,His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (α =32.5 + Fc tagged β=97) kDa. The rh c-MET/Fc heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.

Protein Structure: A DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human c-Met (NP_000236) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Predicted N-Term: Glu 25
Purity > 95 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 μg/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml. Scatchard analysis showed the affinity constant (Kd) of human HGF bound to human c-MET / HGFR was 0.52 nM .
Background Synonyms: AUTS9,c-Met,DFNB97,HGFR,RCCP2
Molecular Weight 45 kDa, 120 kDa
NCBI Accession NP_000236
Pathways RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Met Proto-Oncogene (MET) (Extracellular Domain), (AA 1-932) (Active) protein (Fc Tag,His tag) (ABIN2003239) Met Proto-Oncogene (MET) (Extracellular Domain), (AA 1-932) (Active) protein (Fc Tag,His tag)
Background publications Boccaccio, Comoglio: "Invasive growth: a MET-driven genetic programme for cancer and stem cells." in: Nature reviews. Cancer, Vol. 6, Issue 8, pp. 637-45, 2006

Boccaccio, Comoglio: "Invasive growth: a MET-driven genetic programme for cancer and stem cells." in: Nature reviews. Cancer, Vol. 6, Issue 8, pp. 637-45, 2006 (PubMed).

Birchmeier, Birchmeier, Gherardi, Vande Woude: "Met, metastasis, motility and more." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 12, pp. 915-25, 2003 (PubMed).

Pennacchietti, Michieli, Galluzzo, Mazzone, Giordano, Comoglio: "Hypoxia promotes invasive growth by transcriptional activation of the met protooncogene." in: Cancer cell, Vol. 3, Issue 4, pp. 347-61, 2003 (PubMed).

Weidner, Di Cesare, Sachs, Brinkmann, Behrens, Birchmeier: "Interaction between Gab1 and the c-Met receptor tyrosine kinase is responsible for epithelial morphogenesis." in: Nature, Vol. 384, Issue 6605, pp. 173-6, 1996 (PubMed).

Bottaro, Rubin, Faletto, Chan, Kmiecik, Vande Woude, Aaronson: "Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product." in: Science (New York, N.Y.), Vol. 251, Issue 4995, pp. 802-4, 1991 (PubMed).

Stephenson, Tronick, Aaronson: "Isolation from BALB/c mouse cells of a structural polypeptide of a third endogenous type C virus." in: Cell, Vol. 3, Issue 4, pp. 347-53, 1975 (PubMed).

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