Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (full length), (AA 1-460) (Active) protein (His tag)

Details for Product No. ABIN2003326, Supplier: Log in to see
Protein Name
  • zgc:55553
  • IRAK4
  • ipd1
  • irak-4
  • ny-ran-64
  • ny-ren-64
  • ren64
  • irak4
  • IPD1
  • IRAK-4
  • NY-REN-64
  • REN64
  • 8430405M07Rik
  • 9330209D03Rik
  • interleukin-1 receptor-associated kinase 4
  • Interleukin-1 receptor-associated kinase 4
  • IRAK4
  • irak4
  • LOC100227974
  • Irak4
Protein Characteristics
full length, AA 1-460
3
3
1
1
1
1
1
1
1
1
Origin
Human
10
1
1
Source
Baculovirus infected Insect Cells
4
2
2
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This IRAK4 protein is labelled with His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The recombinant human IRAK4 consists of 478 amino acids and predicts a molecular mass of 53.8 kDa. It migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the full length of human IRAK4 (Q9NWZ3-1) (Met 1-Ser 460) was expressed with a polyhistidine tag at the N-terminus.
Predicted N-Term: Met
Purity > 92 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment The specific activity was determined to be 169 nmol/min/mg using MBP as substrate.
Background Synonyms: IPD1,IRAK-4,NY-REN-64,REN64
Molecular Weight 53.8 kDa, 48 kDa
Research Area Immunology, Innate Immunity, Atherosclerosis, Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer Supplied as sterile 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Expiry Date 12 months
Supplier Images
 image for Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (full length), (AA 1-460) (Active) protein (His tag) (ABIN2003326) Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (full length), (AA 1-460) (Active) protein (His tag)
Background publications Staschke, Dong, Saha, Zhao, Brooks, Hepburn, Xia, Gulen, Kang, Altuntas, Tuohy, Gilmour, Li, Na: "IRAK4 kinase activity is required for Th17 differentiation and Th17-mediated disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 568-77, 2009

Staschke, Dong, Saha, Zhao, Brooks, Hepburn, Xia, Gulen, Kang, Altuntas, Tuohy, Gilmour, Li, Na: "IRAK4 kinase activity is required for Th17 differentiation and Th17-mediated disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 568-77, 2009 (PubMed).

Trumstedt, Eriksson, Lundberg, Yang, Yan, Wigzell, Rottenberg: "Role of IRAK4 and IRF3 in the control of intracellular infection with Chlamydia pneumoniae." in: Journal of leukocyte biology, Vol. 81, Issue 6, pp. 1591-8, 2007 (PubMed).

Strelow, Kollewe, Wesche: "Characterization of Pellino2, a substrate of IRAK1 and IRAK4." in: FEBS letters, Vol. 547, Issue 1-3, pp. 157-61, 2003 (PubMed).

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