Cyclin A1 (CCNA1) (AA 1-465), (full length) (Active) protein (His tag)

Details for Product No. ABIN2003721, Supplier: Log in to see
Protein Name
  • fd14f08
  • wu:fd14f08
  • wu:fi20d12
  • zgc:111869
  • MGC81965
  • cyclin-A1
  • CCNA1
  • MGC132235
  • cyclin-A1-2
  • Cyclin-A1
  • ccna1
  • cyclin-A1-like
  • cyclin A1
  • cyclin-A1-2
  • LOC100147188
  • ccna1
  • CCNA1
  • CYCA1;2
  • Ccna1
  • LOC397885
Protein Characteristics
AA 1-465, full length
2
1
1
1
1
1
1
1
Origin
Human
5
1
1
1
1
Source
Baculovirus infected Insect Cells
3
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This Cyclin A1 protein is labelled with His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The recombinant human CCNA1 consists of 483 amino acids and predicts a molecular mass of 54.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the full length of human CCNA1 isoform a (NP_003905.1) (Met 1-Gln 465) was expressed, with a polyhistidine tag at the N-terminus.
Predicted N-Term: His
Purity > 96 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment 1. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1 . The EC50 of biotinylated human CDK1 is 0.02-0.04 μg/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His . The EC50 of biotinylated human CDK2-His is 0.07-0.15 μg/ml.
Background Synonyms: CT146
Molecular Weight 54.6 kDa, 50 kDa
NCBI Accession NP_003905
Pathways Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Cyclin A1 (CCNA1) (AA 1-465), (full length) (Active) protein (His tag) (ABIN2003721) Cyclin A1 (CCNA1) (AA 1-465), (full length) (Active) protein (His tag)
Background publications Diederichs, Bäumer, Ji, Metzelder, Idos, Cauvet, Wang, Möller, Pierschalski, Gromoll, Schrader, Koeffler, Berdel, Serve, Müller-Tidow: "Identification of interaction partners and substrates of the cyclin A1-CDK2 complex." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33727-41, 2004

Diederichs, Bäumer, Ji, Metzelder, Idos, Cauvet, Wang, Möller, Pierschalski, Gromoll, Schrader, Koeffler, Berdel, Serve, Müller-Tidow: "Identification of interaction partners and substrates of the cyclin A1-CDK2 complex." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33727-41, 2004 (PubMed).

Yang, Müller, Huynh, Fung, Yee, Koeffler: "Functions of cyclin A1 in the cell cycle and its interactions with transcription factor E2F-1 and the Rb family of proteins." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2400-7, 1999 (PubMed).

Yang, Morosetti, Koeffler: "Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines." in: Cancer research, Vol. 57, Issue 5, pp. 913-20, 1997 (PubMed).