Eukaryotic Translation Initiation Factor 2C, 2 (EIF2C2) (AA 1-859), (full length) (Active) protein (His tag)

Details for Product No. ABIN2003923, Supplier: Log in to see
Protein Name
  • EIF2C2
  • T19E23.7
  • T19E23_7
  • argonaute 2
  • Q10
  • Eif2c2
  • Argonaute2
  • eif2c1
  • eif2c2
  • 1110029L17Rik
  • 2310051F07Rik
  • AI225898
  • AL022874
  • AW546247
  • ENSMUSG00000072493
  • Gerp95
  • Gm10365
  • mKIAA4215
  • AGO2
  • AG02
  • AGO 2
  • Ago-2
  • Ago2
  • CG13452
  • CG7439
  • Dm Ago2
  • Dmel\\CG7439
  • ago
  • ago-2
  • ago2
  • dAGO2
  • dAgo2
  • eIF2C2
  • eukaryotic translation initiation factor 2C, 2
  • Argonaute family protein
  • argonaute RISC catalytic component 2
  • argonaute RISC catalytic subunit 2
  • Argonaute 2
  • EIF2C2
  • AGO2
  • Ago2
  • ago2
Protein Characteristics
AA 1-859, full length
2
2
2
1
1
1
1
Origin
Human
8
2
Source
Baculovirus infected Insect Cells
3
2
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This AGO2 protein is labelled with His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The recombinant human AGO2 consists of 877 amino acids and predicts a molecular mass of 99 kDa as estimated by SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the full length of human AGO2 (NP_036286.2) (Met 1-Ala 859) was expressed with a polyhistidine tag at the N-terminus.
Predicted N-Term: Met
Purity > 85 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Human AGO2 (11079-H07B) can bind Let-7a RNA and cleave target RNA (21nt).
Background Synonyms: Argonaute 2,EIF2C2,Q10
Molecular Weight 99 kDa
NCBI Accession NP_036286
Pathways Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4,10 % glycerol,2 mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Eukaryotic Translation Initiation Factor 2C, 2 (EIF2C2) (AA 1-859), (full length) (Active) protein (His tag) (ABIN2003923) Eukaryotic Translation Initiation Factor 2C, 2 (EIF2C2) (AA 1-859), (full length) (Active) protein (His tag)
Product cited in: Bernard, Wang, Tachado: "DICER-ARGONAUTE2 complex in continuous fluorogenic assays of RNA interference enzymes." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120614, 2015 (PubMed).

Zhang, Zuo, Yang, Li, Xue, Zhou, Huang, Zhao, Zhou, Yan, Zhang, Guo, Sun, Guo, Zhang, Fu: "MicroRNA directly enhances mitochondrial translation during muscle differentiation." in: Cell, Vol. 158, Issue 3, pp. 607-19, 2014 (PubMed).

Background publications Qi, Ongusaha, Myllyharju, Cheng, Pakkanen, Shi, Lee, Peng, Shi: "Prolyl 4-hydroxylation regulates Argonaute 2 stability." in: Nature, Vol. 455, Issue 7211, pp. 421-4, 2008

Qi, Ongusaha, Myllyharju, Cheng, Pakkanen, Shi, Lee, Peng, Shi: "Prolyl 4-hydroxylation regulates Argonaute 2 stability." in: Nature, Vol. 455, Issue 7211, pp. 421-4, 2008 (PubMed).

Rand, Petersen, Du, Wang: "Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation." in: Cell, Vol. 123, Issue 4, pp. 621-9, 2005 (PubMed).

Sen, Blau: "Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies." in: Nature cell biology, Vol. 7, Issue 6, pp. 633-6, 2005 (PubMed).

Liu, Carmell, Rivas, Marsden, Thomson, Song, Hammond, Joshua-Tor, Hannon: "Argonaute2 is the catalytic engine of mammalian RNAi." in: Science (New York, N.Y.), Vol. 305, Issue 5689, pp. 1437-41, 2004 (PubMed).

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