Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (full length), (AA 1-631) (Active) protein (His tag)

Details for Product No. ABIN2003953, Supplier: Log in to see
Protein Name
  • ASM
  • Afu4g06640
  • SMPD1
  • ASMASE
  • NPD
  • A-SMase
  • Zn-SMase
  • aSMase
  • sphingomyelin phosphodiesterase
  • acid sphingomyelinase
  • sphingomyelin phosphodiesterase 1, acid lysosomal
  • sphingomyelin phosphodiesterase 1
  • AaeL_AAEL003402
  • ASM
  • AFUA_4G06640
  • SMPD1
  • Smpd1
Protein Characteristics
full length, AA 1-631
1
1
1
1
1
1
1
1
1
1
1
1
Origin
Human
8
2
1
Source
Baculovirus infected Insect Cells
3
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The secreted recombinant human SMPD1 consists of 518 amino acids and predicts a molecular mass of 65 kDa as estimated by SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the full length of human SMPD1 isoform 1 (NP_000534.3) (Met 1-Cys 631) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: His 62
Purity > 94 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Measured by its ability to cleave. 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is >1,000 pmol/min/μg .
Background Synonyms: ASM,ASMASE,NPD
Molecular Weight 65 kDa
NCBI Accession NP_000534
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.1 % OGP, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Liquid. It is shipped out with blue ice.
Expiry Date 12 months
Supplier Images
 image for Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (full length), (AA 1-631) (Active) protein (His tag) (ABIN2003953) Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (full length), (AA 1-631) (Active) protein (His tag)
Background publications Lansmann, Schuette, Bartelsen, Hoernschemeyer, Linke, Weisgerber, Sandhoff: "Human acid sphingomyelinase." in: European journal of biochemistry / FEBS, Vol. 270, Issue 6, pp. 1076-88, 2003 (PubMed).

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