Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165), (full length) protein (GST tag,His tag)

Details for Product No. ABIN2004026, Supplier: Log in to see
Protein Name
  • MGC53491
  • zgc:91795
  • gadd45a
  • GADD45A
  • ddit1
  • gadd45
  • GADD45
  • AA545191
  • Ddit1
  • DDIT1
  • Gadd45
  • growth arrest and DNA-damage-inducible, alpha
  • growth arrest and DNA-damage-inducible, alpha, b
  • growth arrest and DNA damage-inducible protein 45
  • growth arrest and DNA-damage-inducible 45 alpha
  • gadd45a
  • gadd45ab
  • GADD45A
  • GADD45
  • Gadd45a
Protein Characteristics
AA 1-165, full length
11
1
1
1
Origin
Human
11
2
1
1
1
Source
Baculovirus infected Insect Cells
7
3
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This GADD45A protein is labelled with GST tag,His tag.
Application
SDS-PAGE (SDS)
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Characteristics The recombinant human GADD45A/GST chimera consists of 402 amino acids and has a calculated molecular mass of 46.2 kDa. It migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the full length of human GADD45A (P24522) (Met 1-Arg 165) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Predicted N-Term: Met
Purity > 95 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Background Synonyms: DDIT1,GADD45
Molecular Weight 46.2 kDa, 46 kDa
UniProt P24522
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways p53 Signaling, Cell Division Cycle
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % gly
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165), (full length) protein (GST tag,His tag) (ABIN2004026) Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165), (full length) protein (GST tag,His tag)
Background publications Rosemary Siafakas, Richardson: "Growth arrest and DNA damage-45 alpha (GADD45alpha)." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 5, pp. 986-9, 2009

Rosemary Siafakas, Richardson: "Growth arrest and DNA damage-45 alpha (GADD45alpha)." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 5, pp. 986-9, 2009 (PubMed).

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