IL2-Inducible T-Cell Kinase (ITK) (AA 351-619) protein (GST tag,His tag)

Details for Product No. ABIN2007712, Supplier: Log in to see
Protein Name
  • Emt
  • Tcsk
  • Tsk
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • IL2 inducible T cell kinase
  • IL2-inducible T-cell kinase
  • Itk
  • ITK
Protein Characteristics
AA 351-619
4
3
2
1
1
1
1
1
Origin
Mouse (Murine)
12
3
1
Source
Baculovirus infected Insect Cells
6
2
2
2
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This ITK protein is labelled with GST tag,His tag.
Application
SDS-PAGE (SDS)
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Characteristics The secreted recombinant mouse ITK /GST chimera consists of 506 amino acids and has a calculated molecular mass of 58.4 kDa. The recombinant protein migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the mouse ITK / EMT (Q03526) protein kinase domain (Arg 351-Leu 619) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Predicted N-Term: Met
Purity > 95 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment No Kinase Activity
Background Synonyms: Emt,Tcsk,Tsk
Molecular Weight 58.4 kDa, 58 kDa
UniProt Q03526
Research Area Signaling, Tyrosine Kinases
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.5, 10 % gly, 0.5 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for IL2-Inducible T-Cell Kinase (ITK) (AA 351-619) protein (GST tag,His tag) (ABIN2007712) IL2-Inducible T-Cell Kinase (ITK) (AA 351-619) protein (GST tag,His tag)
Background publications Pechloff, Holch, Ferch, Schweneker, Brunner, Kremer, Sparwasser, Quintanilla-Martinez, Zimber-Strobl, Streubel, Gewies, Peschel, Ruland: "The fusion kinase ITK-SYK mimics a T cell receptor signal and drives oncogenesis in conditional mouse models of peripheral T cell lymphoma." in: The Journal of experimental medicine, Vol. 207, Issue 5, pp. 1031-44, 2010

Pechloff, Holch, Ferch, Schweneker, Brunner, Kremer, Sparwasser, Quintanilla-Martinez, Zimber-Strobl, Streubel, Gewies, Peschel, Ruland: "The fusion kinase ITK-SYK mimics a T cell receptor signal and drives oncogenesis in conditional mouse models of peripheral T cell lymphoma." in: The Journal of experimental medicine, Vol. 207, Issue 5, pp. 1031-44, 2010 (PubMed).

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