Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 1-212), (Extracellular Domain) (Active) protein (His tag)

Details for Product No. ABIN2007897, Supplier: Log in to see
Protein Name
  • fpf
  • p55
  • tbp1
  • p55-r
  • tnf-r
  • tnfar
  • tnfr1
  • cd120a
  • tnfr55
  • tnfr60
  • xtnfr1
  • TNFR I
  • TNFRSF1A
  • TNFR1
  • CD120a
  • FPF
  • MS5
  • TBP1
  • TNF-R
  • TNF-R-I
  • TNF-R55
  • TNFAR
  • TNFR1-d2
  • TNFR55
  • TNFR60
  • p55-R
  • p60
  • TNF-R1
  • TNF-alphaR1
  • TNFRI
  • TNFRp55
  • TNFalpha-R1
  • Tnfr-2
  • Tnfr1
  • wu:fj65b10
  • zgc:85914
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor superfamily, member 1a
  • tnfrsf1a
  • TNFRSF1A
  • Tnfrsf1a
Protein Characteristics
AA 1-212, Extracellular Domain
9
5
5
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Origin
Mouse (Murine)
51
11
7
1
1
Source
Human Cells
29
7
7
5
3
3
2
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This TNFRSF1A protein is labelled with His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The secreted recombinant mouse TNFRSF1A consists of 194 amino acids and has a predicted molecular mass of 21.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm TNFRSF1A is approximately 35 kDa due to glycosylation.

Protein Structure: A DNA sequence encoding the mouse TNFRSF1A (NP_ 035739.2) extracellular domain (Met 1-Ala 212) was expressed, with a polyhistidine tag at the C-terminus.
Predicted N-Term: Leu 30
Purity > 96 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment 1. Measured by its binding ability in a functional ELISA.
2. Immobilized human TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28 μg/mL.
3. Immobilized mouse TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13 μg/mL.
4. Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-1.5 μg/mL in the presence of 0.1 ng/mL recombinant mouse TNFα.
Background Synonyms: CD120a,FPF,p55,p55-R,TNF-alphaR1,TNF-R,TNF-R-I,TNF-R1,TNF-R55,TNFalpha-R1,TNFAR,Tnfr-2,Tnfr1,TNFR60,TNFRI,TNFRp55
Molecular Weight 21.8 kDa, 35 kDa
NCBI Accession NP_035739
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 1-212), (Extracellular Domain) (Active) protein (His tag) (ABIN2007897) Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 1-212), (Extracellular Domain) (Active) protein (His tag)
Background publications Chan, Chun, Zheng, Siegel, Bui, Lenardo: "A domain in TNF receptors that mediates ligand-independent receptor assembly and signaling." in: Science (New York, N.Y.), Vol. 288, Issue 5475, pp. 2351-4, 2000 (PubMed).

Peschon, Torrance, Stocking, Glaccum, Otten, Willis, Charrier, Morrissey, Ware, Mohler: "TNF receptor-deficient mice reveal divergent roles for p55 and p75 in several models of inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 943-52, 1998 (PubMed).

Medvedev, Espevik, Ranges, Sundan: "Distinct roles of the two tumor necrosis factor (TNF) receptors in modulating TNF and lymphotoxin alpha effects." in: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9778-84, 1996 (PubMed).

Banner, DArcy, Janes, Gentz, Schoenfeld, Broger, Loetscher, Lesslauer: "Crystal structure of the soluble human 55 kd TNF receptor-human TNF beta complex: implications for TNF receptor activation." in: Cell, Vol. 73, Issue 3, pp. 431-45, 1993 (PubMed).

Lewis, Tartaglia, Lee, Bennett, Rice, Wong, Chen, Goeddel: "Cloning and expression of cDNAs for two distinct murine tumor necrosis factor receptors demonstrate one receptor is species specific." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 7, pp. 2830-4, 1991 (PubMed).