Pepsinogen C (PGC) (AA 1-392) protein (His tag)

Details for Product No. ABIN2009149, Supplier: Log in to see
Protein Name
  • PEPC
  • PGII
  • 2210410L06Rik
  • Upg-1
  • Upg1
  • CPGC
  • gastricsin
  • PG1
  • Pg-1
  • PGNC
  • PGC
  • Gastricsin
  • progastricsin (pepsinogen C)
  • PGC
  • Pgc
Protein Characteristics
AA 1-392
3
2
2
2
2
1
1
1
1
Origin
Rat (Rattus)
12
3
2
1
1
1
1
Source
Human Cells
6
5
3
2
2
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This PGC protein is labelled with His tag.
Application
SDS-PAGE (SDS)
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Characteristics The recombinant rat PGC comprises 387 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the ratPGC is approximately 43 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the rat PGC (NP_579818.1) (Met 1-Val 392) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Ser 17
Purity > 95 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Molecular Weight 42.5 kDa, 43 kDa
NCBI Accession NP_579818
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Pepsinogen C (PGC) (AA 1-392) protein (His tag) (ABIN2009149) Pepsinogen C (PGC) (AA 1-392) protein (His tag)
Background publications Khan, Cherney, Tarasova, James: "Structural characterization of activation 'intermediate 2' on the pathway to human gastricsin." in: Nature structural biology, Vol. 4, Issue 12, pp. 1010-5, 1998 (PubMed).

Moore, Sielecki, Chernaia, Tarasova, James: "Crystal and molecular structures of human progastricsin at 1.62 A resolution." in: Journal of molecular biology, Vol. 247, Issue 3, pp. 466-85, 1995 (PubMed).