Nanog-TAT (AA 1-305), (Lys82Asn-Mutant) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS-PAGE and HPLC analyses.
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- Target
- Nanog-TAT
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-305, Lys82Asn-Mutant
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Characteristics
- AA 1-305 (Lys82Asn), expressed with a C-terminal TAT peptide.
- Purity
- > 95 % by SDS-PAGE and HPLC analyses.
- Sterility
- Sterile filtered
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Sterile Filtered solution contains 10 mM PB, 300 mM NaCl, pH 7.4.
- Handling Advice
- Avoid repeated freezing and thawing cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Recombinant human NANOG-TAT (rhNANOG-TAT) remains stable up to 1-2 weeks at 4 °C from date of receipt. For long term storage, aliquot and store at -20 °C or -80 °C.
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- Target
- Nanog-TAT
- Background
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NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E. coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: None - Molecular Weight
- 36.2 kDa, analyzed by reducing SDS-PAGE.
- UniProt
- Q9H9S0
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