KIT Ligand Protein (KITLG)

Catalog No. ABIN2018375

Product Details

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: The ED50, as determined by the dose-dependant stimulation of human TF-1 cells, is < 2 ng/mL, corresponding to a specific activity of 5x10^5 IU/mg.
The sequence of the first five N-terminal amino acids has been found to be Met-Glu-Gly-Ile-Cys.

Purity: Greater than 95.0 % as determined by the following methods:(a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

Endotoxin Level: < 0.1 ng/μg (IEU/μg) of rHuSCF

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that the lyophilized recombinant human stem cell factor (SCF) be reconstituted in sterile 18 M omega-cm H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.

Buffer: The protein was lyophilized after extensive dialysis against 20 mM HAc-NaAc, pH 4.5, 200 mM NaCl buffer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized recombinant human stem cell factor (SCF) remains stable at room temperature for three weeks, but it is best stored desiccated below -18 °C. Upon reconstitution Recombinant Human Stem Cell Factor (SCF) should be stored at 4 °C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added.

References

Herraiz, Journé, Abdel-Malek, Ghanem, Jiménez-Cervantes, García-Borrón: "Signaling from the human melanocortin 1 receptor to ERK1 and ERK2 mitogen-activated protein kinases involves transactivation of cKIT." in: Molecular endocrinology (Baltimore, Md.), Vol. 25, Issue 1, pp. 138-56, (2011) (PubMed).

Woost, Solchaga, Meyerson, Shankey, Goolsby, Jacobberger: "High-resolution kinetics of cytokine signaling in human CD34/CD117-positive cells in unfractionated bone marrow." in: Blood, Vol. 117, Issue 15, pp. e131-41, (2011) (PubMed).

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: Stem Cell Factor (SCF) (KITLG Products)

Synonyms: Xkl-1 Protein, Xsl Protein, Xsl-1 Protein, Xsl-2 Protein, steel Protein, KITLG Protein, SCF Protein, kitl Protein, kl-1 Protein, mgf Protein, scf Protein, FPH2 Protein, KL-1 Protein, Kitl Protein, MGF Protein, SF Protein, SHEP7 Protein, Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SLF Protein, Sl Protein, blz Protein, contrasted Protein, 710-712 Protein, CSF Protein, KITL Protein, KIT ligand L homeolog Protein, KIT ligand Protein, kit ligand Protein, kitlg.L Protein, KITLG Protein, kitlg Protein, Kitl Protein, Kitlg Protein

Background: Human Stem Cell Factor (SCF), produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18,409 Da. Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. Stem Cell Factor (SCF) stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Synonyms: rHuSCF, SCF

Molecular Weight: 18,409 Da

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway