Oncostatin M (OSM) (AA 26-221) (Active) Protein

Details for Product No. ABIN2039659, Supplier: Log in to see
Protein Name
  • OSM
  • OncoM
  • oncostatin M
  • oncostatin M-like
  • oncostatin-M-like
  • OSM
  • Osm
  • LOC100342491
  • LOC100152038
Protein Characteristics
AA 26-221
6
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Origin
Human
59
9
5
1
Source
HEK293
49
6
3
2
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Sequence AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRR
Characteristics rhOSM tested in Stat3 activation assay using glioblastoma U87 cell line.
ED50: 2.5-3 ng/mL
Purification Produced and purified from Serum Free/ Animal Free media
Purity >99 % , (according to SDS-PAGE stained with SimplyBlue SafeStain, (Invitrogen))
Sterility 0.2 μm filtered
Endotoxin Level Less than 0.1 ng/μg (1 IEU/μg), as measured by LAL method
Background Oncostatin M is a member of the gp130 (IL-6/LIF) family of cytokines. It is naturally processed from 252 amino acid pre-pro OSM polypeptide. Propeptide processing is believed not to be important for OSM receptor binding activity, but may be important for growth-inhibitory activity. Oncostatin M variable cytokine activity depends on the target cell. Oncostatin M was initially identified as a cytokine capable of inhibiting the in vitro growth of melanoma cells and solid tumors (1). It has been subsequently shown to promote proliferation of ovarian cancer cells (2) prostate cancer 22Rv1 cells (3) and AIDS-KS cells (4), up-regulate the ER chaperone Grp78/BiP in the liver cells (5), inhibit proliferation of breast cancer cell lines (6,7) and induce the activation of signaling pathways Jak2, Stat3, and Erk1/2 (8). OSM is normally expressed in macrophages, monocytes, T cells, and dendritic cells (8-10).
Molecular Weight 22.151 kDa
Research Area Immunology, Innate Immunity, Cytokines, Signaling, Receptors
Pathways JAK-STAT Signaling, Negative Regulation of Hormone Secretion
Application Notes 22 kDa, under reducing conditions.
Comment

outstanding bioactivity, superior lot-to-lot reproducibilty in bioactivity assays, sertificate of analysis for every lot.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Spin before opening. For optimal recovery - reconstitute in sterile water at ~0.1 mg/mL at room temperature, after adding water, re-cap the vial and tap gently, ensure to cover all the surfaces inside the vial. Do not mix by vortexing or by extensive pipetting. Let the temperature with gentle agitation for at least 10-15 minutes before aliquoting or using.vial to sit at room
Buffer Dried from 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Handling Advice Avoid repeated freeze-thaw cycles.
Not for use in humans.
Storage -20 °C/-80 °C
Storage Comment To avoid loss of the protein, store the reconstituted protein in aliquots (no smaller than 10 μL) in polypropylene or siliconized tubes. Store dried and reconstituted protein at -20 °C and/or -80 °C.
Supplier Images
 image for Oncostatin M (OSM) (AA 26-221) (Active) Protein (ABIN2039659) Oncostatin M (OSM) (AA 26-221) (Active) protein
Product cited in: Godoy-Tundidor, Cavarretta, Fuchs, Fiechtl, Steiner, Friedbichler, Bartsch, Hobisch, Culig: "Interleukin-6 and oncostatin M stimulation of proliferation of prostate cancer 22Rv1 cells through the signaling pathways of p38 mitogen-activated protein kinase and phosphatidylinositol 3-kinase." in: The Prostate, Vol. 64, Issue 2, pp. 209-16, 2005 (PubMed).

Yin, Chain: "Suppression of lymphokine production in anti-minor histocompatibility antigen responses." in: Cytokine, Vol. 3, Issue 1, pp. 5-11, 1991 (PubMed).

Malik, Kallestad, Gunderson, Austin, Neubauer, Ochs, Marquardt, Zarling, Shoyab, Wei: "Molecular cloning, sequence analysis, and functional expression of a novel growth regulator, oncostatin M." in: Molecular and cellular biology, Vol. 9, Issue 7, pp. 2847-53, 1989 (PubMed).