LIPF Protein (AA 20-398) (His tag)
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- Target See all LIPF Proteins
- LIPF (Lipase, Gastric (LIPF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-398
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This LIPF protein is labelled with His tag.
- Purpose
- Recombinant Human Gastric Triacylglycerol Lipase/LIPF (C-6His)
- Sequence
- LFGKLHPGSP EVTMNISQMI TYWGYPNEEY EVVTEDGYIL EVNRIPYGKK NSGNTGQRPV VFLQHGLLAS ATNWISNLPN NSLAFILADA GYDVWLGNSR GNTWARRNLY YSPDSVEFWA FSFDEMAKYD LPATIDFIVK KAGQKQLHYV GHSQGTTIGF IAFSTNPSLA KRIKTFYALA PVATVKYTKS LINKLRFVPQ SLFKFIFGDK IFYPHNFFDQ FLATEVCSRE MLNLLCSNAL FIICGFDSKN FNTSRLDVYL SHNPAGTSVQ NMFHWTQAVK SGKFQAYDWG SPVQNRMHYD QSQPPYYNVT AMNVPIAVWN GGKDLLADPQ DVGLLLPKLP NLIYHKEIPF YNHLDFIWAM DAPQEVYNDI VSMISEDKKV DHHHHHH
- Characteristics
- Recombinant Human Gastric Triacylglycerol Lipase/LIPF is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu20-Lys398) of Human LIPF fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product LIPF Protein
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Lipase, Gastric (LIPF) (AA 299-398) protein (GST tag)
LIPF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lipase, Gastric (LIPF) (AA 20-398) protein (His tag)Crystallography grade LIPF Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Lipase, Gastric (LIPF) (AA 19-395) protein (His tag)Crystallography grade LIPF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Lipase, Gastric (LIPF) protein (Myc-DYKDDDDK Tag)LIPF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 25 mM TrisHCl, 100 mM glycine, 10 % glycerol, pH 7.3.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- LIPF (Lipase, Gastric (LIPF))
- Alternative Name
- Gastric Triacylglycerol Lipase/LIPF (LIPF Products)
- Synonyms
- fb73g06 Protein, zgc:56632 Protein, wu:fb73g06 Protein, LIPF Protein, GL Protein, HGL Protein, HLAL Protein, 2310051B21Rik Protein, AV082900 Protein, RNLIP Protein, Rnlp Protein, DGL Protein, lipase, gastric Protein, lipase F, gastric type Protein, gastric triacylglycerol lipase Protein, lipf Protein, LIPF Protein, LOC100394535 Protein, Lipf Protein
- Background
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Gastric Triacylglycerol Lipase (LIPF) belongs to the AB hydrolase superfamily. LIPF is an important lipase during the digestion of dietary lipids in cystic fibrosis. LIPF is involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30 % of fat digestion processes occurring in human. LIPF is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. LIPF acts distinct roles in neutral lipid metabolism.
Synonyms: Gastric Triacylglycerol Lipase, GL, Gastric Lipase, LIPF - Molecular Weight
- 44.22 kDa
- UniProt
- P07098
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