UBE2D1 Protein (AA 1-147) (GST tag)

Catalog No. ABIN2115460

Product Details

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Protein Type: Recombinant

Protein Characteristics: AA 1-147

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2D1 protein is labelled with GST tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 D1/UBE2D1/UbcH5a (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMALK RIQKELSDLQ RDPPAHCSAG PVGDDLFHWQ ATIMGPPDSA YQGGVFFLTV HFPTDYPFKP PKIAFTTKIY HPNINSNGSI CLDILRSQWS PALTVSKVLL SICSLLCDPN PDDPLVPDIA QIYKSDKEKY NRHAREWTQK YAM

Characteristics: Recombinant Human Ubiquitin-conjugating enzyme E2 D1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Met147) of Human UBE2D1 fused with a GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES,150 mM NaCl,2 mM DTT,10 % Glycerin pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Alternative Name: Ubiquitin-Conjugating Enzyme E2 D1 (UBE2D1 Products)

Synonyms: E2(17)KB1 Protein, SFT Protein, UBC4/5 Protein, UBCH5 Protein, UBCH5A Protein, ube2d1 Protein, wu:fc16h06 Protein, zgc:73096 Protein, e2(17)kb1 Protein, sft Protein, ubc4/5 Protein, ubch5 Protein, ubch5a Protein, ubiquitin conjugating enzyme E2 D1 Protein, ubiquitin-conjugating enzyme E2D 1 Protein, ubiquitin-conjugating enzyme E2D 1b Protein, ubiquitin conjugating enzyme E2 D1 S homeolog Protein, UBE2D1 Protein, Ube2d1 Protein, ube2d1b Protein, ube2d1.S Protein, ube2d1 Protein

Background: Ubiquitin-conjugating enzyme E2 D1(UBE2D1)belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Synonyms: Ubiquitin-conjugating enzyme E2 D1,Stimulator of Fe transport,SFT,UBC4/5 homolog,UbcH5,Ubiquitin carrier protein D1,Ubiquitin-conjugating enzyme E2(17)KB 1,Ubiquitin-conjugating enzyme E2-17 kDa 1,Ubiquitin-protein ligase D1,SFT, UBC5A, UBCH5, UBCH5A

Molecular Weight: 42.9 kDa

UniProt: P51668

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades