Streptavidin protein (IRDye680RD)

Details for Product No. ABIN2169602, Supplier: Log in to see
Protein Name
Origin
Streptomyces avidinii (S. avidinii)
186
2
1
Source
Streptomyces avidinii
75
49
14
1
1
Protein Type
Native
Purification tag / Conjugate
This Streptavidin protein is labelled with IRDye680RD.
Application
In-Gel Western blotting (gelWB), Light Microscopy (LM), Protein Array (PAr), ELISA, Western Blotting (WB)
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Specificity Biotin
Characteristics IRDye 680RD Streptavidin can be used as a secondary detection reagent for protein arrays, microscopy, In-Gel Western Assays, and Western blotting. To reduce non-specific background staining due to possible aggregates in the solution, centrifuge briefly before use. A final concentration of 0.2 to 1.0 μg/mL (1:1,000 to 1:5,000) is satisfactory for most applications, however, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02 % to 0.1 % final concentration) and Tween 20 (0.1 to 0.2 % final concentration) during the detection incubation step to reduce non-specific background staining.
Research Area Epitope Tags, Tags/Labels, Enzymes
Application Notes Western blot: 1:1,000-1:5,000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 10 mM Phosphate, 183 mM NaCl, 2.7 mM KCl, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Product cited in: Naganathan, Ray-Saha, Park, Tian, Sakmar, Huber: "Multiplex detection of functional g protein-coupled receptors harboring site-specifically modified unnatural amino acids." in: Biochemistry, Vol. 54, Issue 3, pp. 776-86, 2015 (PubMed).