SIRP alpha (AA 31-370) (Active) protein (Strep Tag,His tag)

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Protein Name
Protein Characteristics
AA 31-370
6
Origin
Human
7
Source
HEK-293 Cells
4
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
Strep Tag,His tag
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Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.1 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade HPLC verified
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh CD172a /SIRPA (Catalog # SIA-H5225) at 2 μg/mL can bind CD47-Fc (Catalog # CD7-H5256) with a linear range of 0.1-5 ng/mL .

SDS-PAGE: due to glycosylation.
Background Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Molecular Weight 38.1 kDa
NCBI Accession NP_001035111
UniProt P78324
Research Area Neurology
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for SIRP alpha (AA 31-370) (Active) protein (Strep Tag,His tag) (ABIN2180706) Human SIRP alpha (HPLC-verified), His Tag on SDS-PAGE under reducing (R) condition. T...
Background publications Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, 2001 (PubMed).

Latour, Tanaka, Demeure, Mateo, Rubio, Brown, Maliszewski, Lindberg, Oldenborg, Ullrich, Delespesse, Sarfati et al.: "Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2547-54, 2001 (PubMed).

Stofega, Argetsinger, Wang, Ullrich, Carter-Su: "Negative regulation of growth hormone receptor/JAK2 signaling by signal regulatory protein alpha." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 28222-9, 2000 (PubMed).

Timms, Swanson, Marie-Cardine, Raab, Rudd, Schraven, Neel: "SHPS-1 is a scaffold for assembling distinct adhesion-regulated multi-protein complexes in macrophages." in: Current biology : CB, Vol. 9, Issue 16, pp. 927-30, 1999 (PubMed).