FCGR1A Protein (AA 25-297) (His tag)

Catalog No. ABIN2180821

Product Details

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 25-297

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This FCGR1A protein is labelled with His tag.

Brand: MABSol®

Sequence: AA 25-297

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 31.2 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Chini, Peclat, Warner, Kashyap, Espindola-Netto, de Oliveira, Gomez, Hogan, Tarragó, Puranik, Agorrody, Thompson, Dang, Clarke, Childs, Kanamori, Witte, Vidal, Kirkland, De Cecco, Chellappa et al.: "CD38 ecto-enzyme in immune cells is induced during aging and regulates NAD+ and NMN levels. ..." in: Nature metabolism, Vol. 2, Issue 11, pp. 1284-1304, (2020) (PubMed).

Target Details

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Alternative Name: Fc gamma RI / CD64 (FCGR1A Products)

Synonyms: CD64 Protein, CD64A Protein, FCRI Protein, IGFR1 Protein, FcgammaRI Protein, Fcgr1 Protein, Fcgr1b Protein, AI323638 Protein, AV092959 Protein, IGGHAFC Protein, FCGR1A Protein, Fc fragment of IgG receptor Ia Protein, Fc receptor, IgG, high affinity I Protein, FCGR1A Protein, Fcgr1a Protein, Fcgr1 Protein

Background: Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.

Molecular Weight: 32.3 kDa

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process