Cathepsin B Protein (CTSB) (AA 18-339) (His tag)

Catalog No. ABIN2180942

Product Details

Target: Cathepsin B (CTSB)

Protein Type: Recombinant

Protein Characteristics: AA 18-339

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Cathepsin B protein is labelled with His tag.

Sequence: AA 18-339

Characteristics: rh CTSB is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.7 kDa (pro-form) and 29 kDa (mature-form). The predicted N-terminus is Arg18 (pro-form) or Phe74 (mature-form). Due to glycosylation, The reducing (R) protein migrates as 43 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 8.0

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Hartmann, Lopez Cruz, Alameh, Ho, Rabideau, Pentelute, Bradley, Martchenko: "Characterization of Novel Piperidine-Based Inhibitor of Cathepsin B-Dependent Bacterial Toxins and Viruses." in: ACS infectious diseases, Vol. 4, Issue 8, pp. 1235-1245, (2018) (PubMed).

Zilbermintz, Leonardi, Jeong, Sjodt, McComb, Ho, Retterer, Gharaibeh, Zamani, Soloveva, Bavari, Levitin, West, Bradley, Clubb, Cohen, Gupta, Martchenko: "Identification of agents effective against multiple toxins and viruses by host-oriented cell targeting." in: Scientific reports, Vol. 5, pp. 13476, (2015) (PubMed).

Target Details

Target: Cathepsin B (CTSB)

Alternative Name: Cathepsin B (CTSB Products)

Background: Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds, Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L), In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.

Molecular Weight: 36.7 kDa & 29.5 kDa

NCBI Accession: NP_001899

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades