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EpCAM Protein (AA 24-265) (His tag)

EPCAM Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Catalog No. ABIN2181014
  • Target See all EpCAM (EPCAM) Proteins
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 24-265
    Origin
    • 23
    • 10
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Human
    Source
    • 21
    • 9
    • 5
    • 4
    • 3
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This EpCAM protein is labelled with His tag.
    Sequence
    AA 24-265
    Characteristics
    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    Purity
    >95 % as determined by SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product EPCAM Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
  • Robertson, Lopez-Anton, Gurjar, Khalique, Khalaf, Clerkin, Leydon, Parker-Manuel, Raeside, Payne, Jones, Seymour, Cawood: "Development of a novel mammalian display system for selection of antibodies against membrane proteins." in: The Journal of biological chemistry, Vol. 295, Issue 52, pp. 18436-18448, (2020) (PubMed).

    Abali, Broekmaat, Tibbe, Schasfoort, Zeune, Terstappen: "A microwell array platform to print and measure biomolecules produced by single cells." in: Lab on a chip, Vol. 19, Issue 10, pp. 1850-1859, (2019) (PubMed).

    Stojanović, Baumgartner, van der Velden, Terstappen, Schasfoort: "Modeling single cell antibody excretion on a biosensor." in: Analytical biochemistry, Vol. 504, pp. 1-3, (2017) (PubMed).

    Xu, Lee, Tran, Heibeck, Wang, Yang, Stafford, Steiner, Sato, Hallam, Yin: "Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system." in: mAbs, Vol. 7, Issue 1, pp. 231-42, (2015) (PubMed).

    Zielonka, Empting, Könning, Grzeschik, Krah, Becker, Dickgießer, Kolmar: "The Shark Strikes Twice: Hypervariable Loop 2 of Shark IgNAR Antibody Variable Domains and Its Potential to Function as an Autonomous Paratope." in: Marine biotechnology (New York, N.Y.), Vol. 17, Issue 4, pp. 386-92, (2015) (PubMed).

    Zielonka, Weber, Becker, Doerner, Christmann, Christmann, Uth, Fritz, Schäfer, Steinmann, Empting, Ockelmann, Lierz, Kolmar: "Shark Attack: high affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation." in: Journal of biotechnology, Vol. 191, pp. 236-45, (2014) (PubMed).

  • Target
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    Alternative Name
    EpCAM (EPCAM Products)
    Synonyms
    DIAR5 Protein, EGP-2 Protein, EGP314 Protein, EGP40 Protein, ESA Protein, HNPCC8 Protein, KS1/4 Protein, KSA Protein, M4S1 Protein, MIC18 Protein, MK-1 Protein, TACSTD1 Protein, TROP1 Protein, ECS-1 Protein, ECS1 Protein, ESA1 Protein, M17S1 Protein, EGP Protein, Ep-CAM Protein, sb:cb6 Protein, tacstd Protein, wu:fj17g02 Protein, zgc:110304 Protein, zgc:77119 Protein, tacstd1 Protein, MGC80540 Protein, EPCAM Protein, egp Protein, ksa Protein, m4s1 Protein, mk-1 Protein, cd326 Protein, egp40 Protein, mic18 Protein, trop1 Protein, ep-cam Protein, hegp-2 Protein, co17-1a Protein, ga733-2 Protein, CD326 Protein, Egp314 Protein, EpCAM1 Protein, GA733-2 Protein, Ly74 Protein, Tacsd1 Protein, Tacstd1 Protein, gp40 Protein, epithelial cell adhesion molecule Protein, flotillin 2 Protein, epithelial cell adhesion molecule S homeolog Protein, EPCAM Protein, FLOT2 Protein, epcam Protein, epcam.S Protein, Epcam Protein
    Background
    EpCAM is also known as CO171A, EGP, EGP40,GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
    Molecular Weight
    28.2 kDa
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