FcRn Protein (AA 23-298) (His tag,Strep Tag)

Catalog No. ABIN2181083

Product Details

Target: FcRn (IgG receptor FcRn (FcRn))

Protein Type: Recombinant

Protein Characteristics: AA 23-298

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This FcRn protein is labelled with His tag,Strep Tag.

Brand: MABSol®

Sequence: AA 23-298

Characteristics: Rat FcRn / FCGRT & B2M Heterodimer Protein, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.8 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The predicted N-terminus is Ala 23 (FCGRT) & Ile 21 (B2M). The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14.5 kDa (B2M) respectively due to different glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Kenniston, Taylor, Conley, Cosic, Kopacz, Lindberg, Comeau, Atkins, Bullen, TenHoor, Adelman, Sexton, Edwards, Nixon: "Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody." in: The Journal of biological chemistry, Vol. 292, Issue 42, pp. 17449-17460, (2017) (PubMed).

Target Details

Target: FcRn (IgG receptor FcRn (FcRn))

Alternative Name: FcRn (FcRn Products)

Synonyms: FCRN Protein, alpha-chain Protein, FcRn Protein, Fc fragment of IgG receptor and transporter Protein, Fc receptor, IgG, alpha chain transporter Protein, FCGRT Protein, Fcgrt Protein

Sub Type: Fusionprotein

Background: FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Molecular Weight: 31.8 kDa (FCGRT) & 13.1 kDa (B2M)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process