Glutaredoxin 1 Protein (GRX1) (AA 1-106) (His tag)

Catalog No. ABIN2181166

Product Details

Target: Glutaredoxin 1 (GRX1)

Protein Type: Recombinant

Protein Characteristics: AA 1-106

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Glutaredoxin 1 protein is labelled with His tag.

Sequence: AA 1-106

Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 12.7 kDa. The protein migrates as 12.7 kDa under reducing (R) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5 with 1 mM DTT

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Barinova, Serebryakova, Muronetz, Schmalhausen: "S-glutathionylation of glyceraldehyde-3-phosphate dehydrogenase induces formation of C150-C154 intrasubunit disulfide bond in the active site of the enzyme." in: Biochimica et biophysica acta. General subjects, Vol. 1861, Issue 12, pp. 3167-3177, (2018) (PubMed).

Target Details

Target: Glutaredoxin 1 (GRX1)

Alternative Name: Glutaredoxin 1 (GRX1 Products)

Synonyms: Glrx1 Protein, Grx Protein, grx Protein, wu:fc38f02 Protein, zgc:103707 Protein, GLRXL Protein, Grx1 Protein, TTase Protein, C86710 Protein, D13Wsu156e Protein, grx1 Protein, GLRX Protein, GRX Protein, GRX1 Protein, GLRX1 Protein, TTF Protein, glrx Protein, glutaredoxin Protein, glutaredoxin (thioltransferase) Protein, glutaredoxin Grx1 Protein, NrdH-redoxin Protein, Uncharacterized monothiol glutaredoxin F10D7.3 Protein, glutaredoxin-1 (Grx1) Protein, temporal expression: late Protein, glutaredoxin L homeolog Protein, Glrx Protein, glrx Protein, GLRX Protein, grx1 Protein, AF_RS07750 Protein, F10D7.3 Protein, AFUA_1G06100 Protein, NT01EI_2528 Protein, O2L Protein, glrx.L Protein

Target Type: Viral Protein

Background: Glutaredoxin-1 (GRX1) is also known as Thioltransferase-1 (TTase-1), GLRX, GRX, GLRX1, which belongs to the glutaredoxin family. GRX1 / GLRX contains one glutaredoxin domain, which exists in either a reduced or an oxidized form. GRX1 / GLRX has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase and functions as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.

Molecular Weight: 12.7 kDa

NCBI Accession: NP_001112362

Pathways: Cell RedoxHomeostasis