ErbB2/Her2 Protein (AA 23-652) (Fc Tag)

Catalog No. ABIN2181216

Product Details

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-652

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ErbB2/Her2 protein is labelled with Fc Tag.

Sequence: AA 23-652

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 97.7 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Ding, Gangalum, Galstyan, Fox, Patil, Hubbard, Murali, Ljubimova, Holler: "HER2-positive breast cancer targeting and treatment by a peptide-conjugated mini nanodrug." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 13, Issue 2, pp. 631-639, (2017) (PubMed).

Piechocki, Wu, Jones, Jacob, Gibson, Ethier, Abrams, Yagita, Venuprasad, Wei: "Induction of proapoptotic antibodies to triple-negative breast cancer by vaccination with TRAIL death receptor DR5 DNA." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 11, pp. 2562-72, (2012) (PubMed).

Target Details

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: Her2 (ErbB2/Her2 Products)

Synonyms: CD340 Protein, HER-2 Protein, HER-2/neu Protein, HER2 Protein, MLN 19 Protein, NEU Protein, NGL Protein, TKR1 Protein, Erbb-2 Protein, Neu Protein, c-erbB2 Protein, c-neu Protein, mKIAA3023 Protein, wu:fv70f10 Protein, zgc:63601 Protein, erb2 Protein, erb-b2 receptor tyrosine kinase 2 Protein, ERBB2 Protein, Erbb2 Protein, erbb2 Protein

Background: Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30 % of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .

Molecular Weight: 96.0 kDa

NCBI Accession: NP_004439

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development