HMGB1 Protein (AA 1-215) (His tag)
-
- Target See all HMGB1 Proteins
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-215
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HMGB1 protein is labelled with His tag.
- Sequence
- AA 1-215
- Characteristics
- This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.7 kDa. The protein migrates as 28 kDa and 32 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
- Purity
- >90 % as determined by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product HMGB1 Protein
-
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag,Biotin)HMGB1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (Non-oxidizable) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 Origin: Human Host: Hi-5 Cells Recombinant > 90 % by SDS - PAGE SDS
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 2-215) protein (His tag)Crystallography grade HMGB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
High Mobility Group Box 1 (HMGB1) (AA 2-215) protein (His tag)Crystallography grade HMGB1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
-
- Target
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Alternative Name
- HMGB1 (HMGB1 Products)
- Background
- High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.
- Molecular Weight
- 26.8 kDa
- NCBI Accession
- NP_002119
- Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
-
