Maltose Binding Protein (MBP) (AA 27-396) (Active) Protein

Details for Product No. ABIN2181500, Supplier: Log in to see
Protein Name
Protein Characteristics
AA 27-396
2
1
Origin
Others
6
1
Source
Escherichia coli (E. coli)
4
Protein Type
Recombinant
Biological Activity
Active
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Characteristics This protein carries no "tag". The protein has a calculated MW of 40.7 kDa. The protein migrates as 45 kDa on a SDS-PAGE gel under reducing (R) condition.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kDa. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.
Molecular Weight 40.7 kDa
Gene ID 17
NCBI Accession ZP_11648096
Research Area Complement Factors, Receptors, Serum/Plasma Proteins, Inflammation
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 20 mM tris, 200 mM NaCl, 1 mM EDTA, 10 mM maltose, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Maltose Binding Protein (MBP) (AA 27-396) (Active) Protein (ABIN2181500) Maltose Binding Protein on SDS-PAGE under reducing (R) condition. The gel was stained...
Background publications Quiocho, Spurlino, Rodseth: "Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor." in: Structure (London, England : 1993), Vol. 5, Issue 8, pp. 997-1015, 1997 (PubMed).

Duplay, Bedouelle, Fowler, Zabin, Saurin, Hofnung: "Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12." in: The Journal of biological chemistry, Vol. 259, Issue 16, pp. 10606-13, 1984 (PubMed).