MME Protein (AA 52-750)

Catalog No. ABIN2181505

Product Details

Target: MME (Membrane Metallo-Endopeptidase (MME))

Protein Type: Recombinant

Protein Characteristics: AA 52-750

Origin: Human

Source: HEK-293 Cells

Sequence: AA 52-750

Characteristics: rhCD10, with Gly-Pro at the N- terminus, has a calculated MW of 80 kDa. The predicted N-terminus is Tyr 52. The reducing (R) protein migrates as 90-100 kDa due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 100 mM NaCl, pH 8.0

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Willard, Barrow, Zraika: "Improved glycaemia in high-fat-fed neprilysin-deficient mice is associated with reduced DPP-4 activity and increased active GLP-1 levels." in: Diabetologia, Vol. 60, Issue 4, pp. 701-708, (2017) (PubMed).

Target Details

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: Neprilysin (MME Products)

Synonyms: CALLA Protein, CD10 Protein, NEP Protein, SFE Protein, 6030454K05Rik Protein, C85356 Protein, Nep Protein, neprilysin Protein, membrane metalloendopeptidase Protein, membrane metallo endopeptidase Protein, membrane metallo-endopeptidase Protein, MME Protein, Mme Protein

Target Type: Chemical

Background: Cluster of differentiation 10 (CD10) is also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, and common acute lymphoblastic leukemia antigen (CALLA), is a 90-110- kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. CD20 is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer's disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL).

Molecular Weight: 80.0 kDa

NCBI Accession: NP_000893

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration