KIT Ligand Protein (KITLG) (AA 26-189) (His tag)

Catalog No. ABIN2181732

Product Details

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 26-189

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This KIT Ligand protein is labelled with His tag.

Sequence: AA 26-189

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.1 kDa. The protein migrates as 19-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: SCF (KITLG Products)

Synonyms: Xkl-1 Protein, Xsl Protein, Xsl-1 Protein, Xsl-2 Protein, steel Protein, KITLG Protein, SCF Protein, kitl Protein, kl-1 Protein, mgf Protein, scf Protein, FPH2 Protein, KL-1 Protein, Kitl Protein, MGF Protein, SF Protein, SHEP7 Protein, Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SLF Protein, Sl Protein, blz Protein, contrasted Protein, 710-712 Protein, CSF Protein, KITL Protein, KIT ligand L homeolog Protein, KIT ligand Protein, kit ligand Protein, kitlg.L Protein, KITLG Protein, kitlg Protein, Kitl Protein, Kitlg Protein

Background: Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.

Molecular Weight: 19.5 kDa

NCBI Accession: NP_038626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway