TNFRSF10A Protein (AA 24-239) (Fc Tag)

Catalog No. ABIN2181864

Product Details

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Protein Type: Recombinant

Protein Characteristics: AA 24-239

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF10A protein is labelled with Fc Tag.

Sequence: AA 24-239

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49 kDa. The protein migrates as 47-49 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Mettler Izquierdo, Varela, Park, Collarini, Lu, Pramanick, Rucker, Lopalco, Etches, Harriman: "High-efficiency antibody discovery achieved with multiplexed microscopy." in: Microscopy (Oxford, England), Vol. 65, Issue 4, pp. 341-52, (2018) (PubMed).

Target Details

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: TRAIL R1 (TNFRSF10A Products)

Synonyms: APO2 Protein, CD261 Protein, DR4 Protein, TRAILR-1 Protein, TRAILR1 Protein, TNFRSF10A Protein, TNF receptor superfamily member 10a Protein, tumor necrosis factor receptor superfamily member 26 Protein, tumor necrosis factor receptor superfamily, member 10a Protein, TNFRSF10A Protein, LOC510798 Protein

Background: Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily. TRAILR1 / TNFRSF10A contains 1 death domain and 3 TNFR-Cys repeats. TNFRSF10A / DR4 is widely expressed and high levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells. APO2 / TNFRSF10A is receptor for the cytotoxic ligand TNFSF10 / TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAILR-1 / DR4 / CD261 promotes the activation of NF-kappa-B.

Molecular Weight: 49.0 kDa

NCBI Accession: NP_003835

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity