TNFRSF10B Protein (AA 56-182) (Fc Tag)

Catalog No. ABIN2181868

Product Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 56-182

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF10B protein is labelled with Fc Tag.

Sequence: AA 56-182

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 40.5 kDa. The protein migrates as 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM NaCl, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Mettler Izquierdo, Varela, Park, Collarini, Lu, Pramanick, Rucker, Lopalco, Etches, Harriman: "High-efficiency antibody discovery achieved with multiplexed microscopy." in: Microscopy (Oxford, England), Vol. 65, Issue 4, pp. 341-52, (2018) (PubMed).

Target Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TRAIL R2 (TNFRSF10B Products)

Synonyms: TNFRSF10B Protein, dr5 Protein, cd262 Protein, killer Protein, trick2 Protein, trickb Protein, xDR-M1 Protein, xDR-M2 Protein, ztnfr9 Protein, trailr2 Protein, trick2a Protein, trick2b Protein, CD262 Protein, DR5 Protein, KILLER Protein, KILLER/DR5 Protein, TRAIL-R2 Protein, TRAILR2 Protein, TRICK2 Protein, TRICK2A Protein, TRICK2B Protein, TRICKB Protein, ZTNFR9 Protein, Ly98 Protein, MK Protein, tumor necrosis factor receptor superfamily, member 10b Protein, tumor necrosis factor receptor superfamily member 10b S homeolog Protein, tumor necrosis factor receptor superfamily member 10b L homeolog Protein, tumor necrosis factor receptor superfamily member 10b Protein, TNF receptor superfamily member 10b Protein, Tnfrsf10b Protein, TNFRSF10B Protein, tnfrsf10b.S Protein, tnfrsf10b.L Protein, tnfrsf10b Protein

Background: Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues, very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.

Molecular Weight: 40.4 kDa

NCBI Accession: NP_003833

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity