Botulinum C3 Enzyme (Active) Protein

Catalog No. ABIN2452165

Product Details

Target: Botulinum C3 Enzyme

Protein Type: Native

Biological Activity: Active

Origin: Clostridium botulinum

Source: Clostridium botulinum

Application: Functional Studies (Func)

Purity: > 90 % purity by SDS-PAGE (CBB staining)

Application Details

Application Notes: 1) For the studies on the Rho-dependent signaling pathways in animal cells in vivo and in vitro.
2) ADP-ribosylation of small GTP proteins, such as Rho and Rac proteins.
Activity: Stimulation of human keratinocytes in culture was observed at 50 ng/mL.

Comment:

No toxic effect was observed in two mice injected with 5 ug of C3 enzyme, showing little contamination of lethal neurotoxins from the botulinus culuture in this preparation.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.33 mg/mL

Buffer: 5 mM sodium phosphate buffer ( pH 6.0), 50 % glycerol

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References

Fiorentini, Gauthier, Donelli, Boquet: "Bacterial toxins and the Rho GTP-binding protein: what microbes teach us about cell regulation." in: Cell death and differentiation, Vol. 5, Issue 9, pp. 720-8, (1999) (PubMed).

Moriishi, Syuto, Yokosawa, Oguma, Saito: "Purification and characterization of ADP-ribosyltransferases (exoenzyme C3) of Clostridium botulinum type C and D strains." in: Journal of bacteriology, Vol. 173, Issue 19, pp. 6025-9, (1991) (PubMed).

Target Details

Target: Botulinum C3 Enzyme

Background: This product was highly purified from the supernatant of the culture of Clostridium botulinum type C by cation exchange, hydroxyapatite and gel filtration columns. This toxin ADP-ribosylates Rho and Rac, which are low-molecular weight GTP-binding proteins of animal cells, and interrupts the downstream signal transduction pathways. The molecular weight is 24 kDa.

UniProt: P15879