Dermonecrotizing Toxin (Active) Protein

Details for Product No. ABIN2452168, Supplier: Log in to see
Protein Name
Origin
Bordetella bronchiseptica
Source
Bordetella bronochiseptica
Protein Type
Native
Biological Activity
Active
Application
Functional Studies (Func)
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Characteristics Activity: DNT at 5 ng/mL induces ballooning morphological change in cultured mouse osteoblastic cell line MC3T3-E1.
Purity > 95 % purity by SDS-PAGE (CBB staining)
Sterility Sterile filtered
Background Dermonecrotic toxin (DNT) is a common toxin produced by pathogenic bacteria belonging to the genus Bordetella, such as B. pertussis, B. parapertussis, and B. bronchiseptica. This toxin activates small GTP-binding proteins such as Rho, Rac and Cdc42 through deamidation or polyamination for downstream signaling pathways. DNT was purified from B. bronchiseptica strain S798 by propriety chromatography and differential precipitation method.
UniProt Q7WGE1
Application Notes 1) For the studies of the signaling pathways mediated by small GTP-binding proteins.
2) Antigen to be used for detection of infection of Bordetella by immunological method.
Restrictions For Research Use only
Format Liquid
Concentration 0.53 mg/mL
Buffer 50 mM sodium phosphate buffer ( pH 7.2), 1 M urea, 0.3 M sodium sulfate
Handling Advice Do not freeze!
Storage 4 °C
Supplier Images
SDS-PAGE (SDS) image for Dermonecrotizing Toxin (Active) Protein (ABIN2452168) Dermonecrotizing Toxin (Active) protein
SDS-PAGE (SDS) image for Dermonecrotizing Toxin (Active) Protein (ABIN2452168) Dermonecrotizing Toxin (Active) protein (Image 2)
SDS-PAGE (SDS) image for Dermonecrotizing Toxin (Active) Protein (ABIN2452168) Dermonecrotizing Toxin (Active) protein (Image 3)
Product cited in: Masuda, Betancourt, Matsuzawa, Kashimoto, Takao, Shimonishi, Horiguchi: "Activation of rho through a cross-link with polyamines catalyzed by Bordetella dermonecrotizing toxin." in: The EMBO journal, Vol. 19, Issue 4, pp. 521-30, 2000 (PubMed).

Horiguchi, Nakai, Kume: "Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin." in: FEMS microbiology letters, Vol. 54, Issue 1-3, pp. 39-43, 1990 (PubMed).