POLK Protein
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- Target See all POLK Proteins
- POLK (Polymerase (DNA Directed) kappa (POLK))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- ELISA, Western Blotting (WB), Functional Studies (Func)
- Characteristics
- This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase κ (1-560 aa), and highly purified by several steps of chromatography. The product is catalytically active and its molecular weight is 65 kD. Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold.
- Purity
- > 90 % by SDS-PAGE (CBB staining)
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- Application Notes
- Other applications are not tested.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 3.2 mg/mL
- Buffer
- 0.2 M NaCl, 10 mM sodium phosphate buffer ( pH 7.0), 50 % glycerol
- Storage
- -20 °C/-80 °C
- Storage Comment
- Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
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Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39678-84, (2001) (PubMed).
: "Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa." in: Genes & development, Vol. 14, Issue 13, pp. 1589-94, (2000) (PubMed).
: "The metabolism of oleic, linoleic and linolenic acids by sheep with reference to their effects on methane production." in: The British journal of nutrition, Vol. 20, Issue 2, pp. 349-62 (PubMed).
: "
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Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39678-84, (2001) (PubMed).
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- Target
- POLK (Polymerase (DNA Directed) kappa (POLK))
- Alternative Name
- DNA Polymerase kappa (POLK Products)
- Synonyms
- DINB1 Protein, DINP Protein, POLQ Protein, Dinb1 Protein, POLK Protein, si:ch211-254o18.3 Protein, DNA polymerase kappa Protein, polymerase (DNA directed) kappa Protein, polymerase (DNA directed), kappa Protein, polymerase (DNA directed) kappa L homeolog Protein, POLK Protein, Polk Protein, polk Protein, Tb11.01.0080 Protein, Tb11.01.0010 Protein, Tb11.01.0020 Protein, Tb11.01.0030 Protein, Tb11.01.0040 Protein, Tb11.01.0050 Protein, Tb11.12.0001 Protein, Tb11.12.0002 Protein, Tb11.12.0003 Protein, polk.L Protein, polk-1 Protein
- Background
- Mammalian DNA polymerase κ, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis. Human DNA polymerase κ copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10-3 and 2 x 10-3, respectively. These error rates are high when compared to those of most other DNA polymerases. DNA polymerase κ has important role in the mutagenic bypass of certain types of DNA lesions.
- UniProt
- Q9UBT6
- Pathways
- DNA Damage Repair
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