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POLK Protein

POLK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS-PAGE (CBB staining) ELISA, WB, Func Active
Catalog No. ABIN2452172
  • Target See all POLK Proteins
    POLK (Polymerase (DNA Directed) kappa (POLK))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 4
    • 1
    Human
    Source
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    ELISA, Western Blotting (WB), Functional Studies (Func)
    Characteristics
    This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase κ (1-560 aa), and highly purified by several steps of chromatography. The product is catalytically active and its molecular weight is 65 kD. Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold.
    Purity
    > 90 % by SDS-PAGE (CBB staining)
  • Application Notes
    Other applications are not tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    3.2 mg/mL
    Buffer
    0.2 M NaCl, 10 mM sodium phosphate buffer ( pH 7.0), 50 % glycerol
    Storage
    -20 °C/-80 °C
    Storage Comment
    Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
  • Ohashi, Bebenek, Matsuda, Feaver, Gerlach, Friedberg, Ohmori, Kunkel: "Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39678-84, (2001) (PubMed).

    Ohashi, Ogi, Kusumoto, Iwai, Masutani, Hanaoka, Ohmori: "Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa." in: Genes & development, Vol. 14, Issue 13, pp. 1589-94, (2000) (PubMed).

    Czerkawski, Blaxter, Wainman: "The metabolism of oleic, linoleic and linolenic acids by sheep with reference to their effects on methane production." in: The British journal of nutrition, Vol. 20, Issue 2, pp. 349-62 (PubMed).

  • Target
    POLK (Polymerase (DNA Directed) kappa (POLK))
    Alternative Name
    DNA Polymerase kappa (POLK Products)
    Synonyms
    DINB1 Protein, DINP Protein, POLQ Protein, Dinb1 Protein, POLK Protein, si:ch211-254o18.3 Protein, DNA polymerase kappa Protein, polymerase (DNA directed) kappa Protein, polymerase (DNA directed), kappa Protein, polymerase (DNA directed) kappa L homeolog Protein, POLK Protein, Polk Protein, polk Protein, Tb11.01.0080 Protein, Tb11.01.0010 Protein, Tb11.01.0020 Protein, Tb11.01.0030 Protein, Tb11.01.0040 Protein, Tb11.01.0050 Protein, Tb11.12.0001 Protein, Tb11.12.0002 Protein, Tb11.12.0003 Protein, polk.L Protein, polk-1 Protein
    Background
    Mammalian DNA polymerase κ, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis. Human DNA polymerase κ copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10-3 and 2 x 10-3, respectively. These error rates are high when compared to those of most other DNA polymerases. DNA polymerase κ has important role in the mutagenic bypass of certain types of DNA lesions.
    UniProt
    Q9UBT6
    Pathways
    DNA Damage Repair
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