Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

RuvB (Active) Protein

Origin: E. coli Host: Escherichia coli (E. coli) Native SDS, Func, ELISA, WB Active
Catalog No. ABIN2452184
  • Target
    RuvB
    Protein Type
    Native
    Biological Activity
    Active
    Origin
    E. coli
    Source
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
    Characteristics
    Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvB protein is over 90 % by SDS-PAGE (CBB staining) pure.
  • Application Notes
    1) Functional studies in vitro,RuvA and RuvB form a complex that promotes Holiday junction (a recombination intermediatae) branch-migration by using ATP hydrolysis energy. RuvB also has ATPase activity which is stimulated by RuvA and DNA.
    2) SDS-PAGE (0. 2 g/lane)
    3) Standard antigen for western blotting and ELISA
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
    Storage
    -20 °C/-80 °C
    Storage Comment
    Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
  • Shinagawa, Iwasaki: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).

    Iwasaki, Takahagi, Nakata, Shinagawa: "Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration." in: Genes & development, Vol. 6, Issue 11, pp. 2214-20, (1992) (PubMed).

  • Target
    RuvB
    Background
    E. coli RuvB protein forms a complex with RuvA protein at the late stage of homologous recombination and recombination repair and binds specifically to the Holliday structure which is the intermediate of recombination, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. RuvB forms a hexamer ring structure and surrounds the double chain DNA and covers RuvA tetramer bound to the Holliday junction from both sides. RuvB is a DNA motor protein which possesses the ATPase activity, activated by DNA and RuvA protein. Its molecular weight is 37 kD and forms a dimer in solution in the physiological condition. .
    UniProt
    P0A812
You are here:
Support