RuvC Protein
RuvC
Origin: E. coli
Host: Escherichia coli (E. coli)
Native
SDS, Func, ELISA, WB
Active
(3 references)-
- Target See all RuvC products
- RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
- Protein Type
- Native
- Biological Activity
- Active
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
- Characteristics
- Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
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Crossover Junction Endodeoxyribonuclease RuvC (RuvC) (AA 2-173) protein (His-SUMO Tag)
RuvC Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
-
1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
2) SDS-PAGE (0. 2 g/lane)
3) Standard antigen for western blotting and ELISA - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
- Storage
- -20 °C/-80 °C
- Storage Comment
- Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
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-
: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).
: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).
: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." in: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, (1992) (PubMed).
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: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).
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- Target
- RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
- Alternative Name
- RuvC (RuvC Products)
- Synonyms
- ECK1864 Protein, JW1852 Protein, Crossover junction endodeoxyribonuclease RuvC Protein, ruvC Protein
- Background
- E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
- UniProt
- P0A814
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